A PCR-Based Method to Construct Lentiviral Vector Expressing Double Tough Decoy for miRNA Inhibition

DNA vector-encoded Tough Decoy (TuD) miRNA inhibitor is attracting increased attention due to its high efficiency in miRNA suppression. The current methods used to construct TuD vectors are based on synthesizing long oligonucleotides (~90 mer), which have been costly and problematic because of mutat...

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Detalles Bibliográficos
Autores principales: Qiu, Huiling, Zhong, Jiasheng, Luo, Lan, Liu, Nian, Kang, Kang, Qu, Junle, Peng, Wenda, Gou, Deming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666662/
https://www.ncbi.nlm.nih.gov/pubmed/26624995
http://dx.doi.org/10.1371/journal.pone.0143864

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666662/
https://www.ncbi.nlm.nih.gov/pubmed/26624995
http://dx.doi.org/10.1371/journal.pone.0143864

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