Continuously Tunable Nucleic Acid Hybridization Probes

In silico designed nucleic acid probes and primers often fail to achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. Here, we present a novel, on-the-fly method of tuning probe af...

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Detalles Bibliográficos
Autores principales: Wu, Lucia R., Wang, J. Sherry, Fang, John Z., Reiser, Emily, Pinto, Alessandro, Pekker, Irena, Boykin, Richard, Ngouenet, Celine, Webster, Philippa J., Beechem, Joseph, Zhang, David Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666732/
https://www.ncbi.nlm.nih.gov/pubmed/26480474
http://dx.doi.org/10.1038/nmeth.3626

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666732/
https://www.ncbi.nlm.nih.gov/pubmed/26480474
http://dx.doi.org/10.1038/nmeth.3626

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