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RNA-Seq workflow: gene-level exploratory analysis and differential expression

Here we walk through an end-to-end gene-level RNA-Seq differential expression workflow using Bioconductor packages. We will start from the FASTQ files, show how these were aligned to the reference genome, and prepare a count matrix which tallies the number of RNA-seq reads/fragments within each gene...

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Detalles Bibliográficos
Autores principales: Love, Michael I., Anders, Simon, Kim, Vladislav, Huber, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670015/
https://www.ncbi.nlm.nih.gov/pubmed/26674615
http://dx.doi.org/10.12688/f1000research.7035.1