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DNA Damage Response Proteins and Oxygen Modulate Prostaglandin E(2) Growth Factor Release in Response to Low and High LET Ionizing Radiation

Common cancer therapies employ chemicals or radiation that damage DNA. Cancer and normal cells respond to DNA damage by activating complex networks of DNA damage sensor, signal transducer, and effector proteins that arrest cell cycle progression, and repair damaged DNA. If damage is severe enough, t...

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Autores principales: Allen, Christopher P., Tinganelli, Walter, Sharma, Neelam, Nie, Jingyi, Sicard, Cory, Natale, Francesco, King, Maurice, Keysar, Steven B., Jimeno, Antonio, Furusawa, Yoshiya, Okayasu, Ryuichi, Fujimori, Akira, Durante, Marco, Nickoloff, Jac A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670845/
https://www.ncbi.nlm.nih.gov/pubmed/26697402
http://dx.doi.org/10.3389/fonc.2015.00260
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author Allen, Christopher P.
Tinganelli, Walter
Sharma, Neelam
Nie, Jingyi
Sicard, Cory
Natale, Francesco
King, Maurice
Keysar, Steven B.
Jimeno, Antonio
Furusawa, Yoshiya
Okayasu, Ryuichi
Fujimori, Akira
Durante, Marco
Nickoloff, Jac A.
author_facet Allen, Christopher P.
Tinganelli, Walter
Sharma, Neelam
Nie, Jingyi
Sicard, Cory
Natale, Francesco
King, Maurice
Keysar, Steven B.
Jimeno, Antonio
Furusawa, Yoshiya
Okayasu, Ryuichi
Fujimori, Akira
Durante, Marco
Nickoloff, Jac A.
author_sort Allen, Christopher P.
collection PubMed
description Common cancer therapies employ chemicals or radiation that damage DNA. Cancer and normal cells respond to DNA damage by activating complex networks of DNA damage sensor, signal transducer, and effector proteins that arrest cell cycle progression, and repair damaged DNA. If damage is severe enough, the DNA damage response (DDR) triggers programed cell death by apoptosis or other pathways. Caspase 3 is a protease that is activated upon damage and triggers apoptosis, and production of prostaglandin E(2) (PGE(2)), a potent growth factor that can enhance growth of surviving cancer cells leading to accelerated tumor repopulation. Thus, dying tumor cells can promote growth of surviving tumor cells, a pathway aptly named Phoenix Rising. In the present study, we surveyed Phoenix Rising responses in a variety of normal and established cancer cell lines, and in cancer cell lines freshly derived from patients. We demonstrate that IR induces a Phoenix Rising response in many, but not all cell lines, and that PGE(2) production generally correlates with enhanced growth of cells that survive irradiation, and of unirradiated cells co-cultured with irradiated cells. We show that PGE(2) production is stimulated by low and high LET ionizing radiation, and can be enhanced or suppressed by inhibitors of key DDR proteins. PGE(2) is produced downstream of caspase 3 and the cyclooxygenases COX1 and COX2, and we show that the pan COX1–2 inhibitor indomethacin blocks IR-induced PGE(2) production in the presence or absence of DDR inhibitors. COX1–2 require oxygen for catalytic activity, and we further show that PGE(2) production is markedly suppressed in cells cultured under low (1%) oxygen concentration. Thus, Phoenix Rising is most likely to cause repopulation of tumors with relatively high oxygen, but not in hypoxic tumors. This survey lays a foundation for future studies to further define tumor responses to radiation and inhibitors of the DDR and Phoenix Rising to enhance the efficacy of radiotherapy with the ultimate goal of precision medicine informed by deep understanding of specific tumor responses to radiation and adjunct chemotherapy targeting key factors in the DDR and Phoenix Rising pathways.
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spelling pubmed-46708452015-12-22 DNA Damage Response Proteins and Oxygen Modulate Prostaglandin E(2) Growth Factor Release in Response to Low and High LET Ionizing Radiation Allen, Christopher P. Tinganelli, Walter Sharma, Neelam Nie, Jingyi Sicard, Cory Natale, Francesco King, Maurice Keysar, Steven B. Jimeno, Antonio Furusawa, Yoshiya Okayasu, Ryuichi Fujimori, Akira Durante, Marco Nickoloff, Jac A. Front Oncol Oncology Common cancer therapies employ chemicals or radiation that damage DNA. Cancer and normal cells respond to DNA damage by activating complex networks of DNA damage sensor, signal transducer, and effector proteins that arrest cell cycle progression, and repair damaged DNA. If damage is severe enough, the DNA damage response (DDR) triggers programed cell death by apoptosis or other pathways. Caspase 3 is a protease that is activated upon damage and triggers apoptosis, and production of prostaglandin E(2) (PGE(2)), a potent growth factor that can enhance growth of surviving cancer cells leading to accelerated tumor repopulation. Thus, dying tumor cells can promote growth of surviving tumor cells, a pathway aptly named Phoenix Rising. In the present study, we surveyed Phoenix Rising responses in a variety of normal and established cancer cell lines, and in cancer cell lines freshly derived from patients. We demonstrate that IR induces a Phoenix Rising response in many, but not all cell lines, and that PGE(2) production generally correlates with enhanced growth of cells that survive irradiation, and of unirradiated cells co-cultured with irradiated cells. We show that PGE(2) production is stimulated by low and high LET ionizing radiation, and can be enhanced or suppressed by inhibitors of key DDR proteins. PGE(2) is produced downstream of caspase 3 and the cyclooxygenases COX1 and COX2, and we show that the pan COX1–2 inhibitor indomethacin blocks IR-induced PGE(2) production in the presence or absence of DDR inhibitors. COX1–2 require oxygen for catalytic activity, and we further show that PGE(2) production is markedly suppressed in cells cultured under low (1%) oxygen concentration. Thus, Phoenix Rising is most likely to cause repopulation of tumors with relatively high oxygen, but not in hypoxic tumors. This survey lays a foundation for future studies to further define tumor responses to radiation and inhibitors of the DDR and Phoenix Rising to enhance the efficacy of radiotherapy with the ultimate goal of precision medicine informed by deep understanding of specific tumor responses to radiation and adjunct chemotherapy targeting key factors in the DDR and Phoenix Rising pathways. Frontiers Media S.A. 2015-12-07 /pmc/articles/PMC4670845/ /pubmed/26697402 http://dx.doi.org/10.3389/fonc.2015.00260 Text en Copyright © 2015 Allen, Tinganelli, Sharma, Nie, Sicard, Natale, King, Keysar, Jimeno, Furusawa, Okayasu, Fujimori, Durante and Nickoloff. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Allen, Christopher P.
Tinganelli, Walter
Sharma, Neelam
Nie, Jingyi
Sicard, Cory
Natale, Francesco
King, Maurice
Keysar, Steven B.
Jimeno, Antonio
Furusawa, Yoshiya
Okayasu, Ryuichi
Fujimori, Akira
Durante, Marco
Nickoloff, Jac A.
DNA Damage Response Proteins and Oxygen Modulate Prostaglandin E(2) Growth Factor Release in Response to Low and High LET Ionizing Radiation
title DNA Damage Response Proteins and Oxygen Modulate Prostaglandin E(2) Growth Factor Release in Response to Low and High LET Ionizing Radiation
title_full DNA Damage Response Proteins and Oxygen Modulate Prostaglandin E(2) Growth Factor Release in Response to Low and High LET Ionizing Radiation
title_fullStr DNA Damage Response Proteins and Oxygen Modulate Prostaglandin E(2) Growth Factor Release in Response to Low and High LET Ionizing Radiation
title_full_unstemmed DNA Damage Response Proteins and Oxygen Modulate Prostaglandin E(2) Growth Factor Release in Response to Low and High LET Ionizing Radiation
title_short DNA Damage Response Proteins and Oxygen Modulate Prostaglandin E(2) Growth Factor Release in Response to Low and High LET Ionizing Radiation
title_sort dna damage response proteins and oxygen modulate prostaglandin e(2) growth factor release in response to low and high let ionizing radiation
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670845/
https://www.ncbi.nlm.nih.gov/pubmed/26697402
http://dx.doi.org/10.3389/fonc.2015.00260
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