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Yokukansan Increases 5-HT(1A) Receptors in the Prefrontal Cortex and Enhances 5-HT(1A) Receptor Agonist-Induced Behavioral Responses in Socially Isolated Mice

The traditional Japanese medicine yokukansan has an anxiolytic effect, which occurs after repeated administration. In this study, to investigate the underlying mechanisms, we examined the effects of repeated yokukansan administration on serotonin 1A (5-HT(1A)) receptor density and affinity and its e...

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Autores principales: Ueki, Toshiyuki, Mizoguchi, Kazushige, Yamaguchi, Takuji, Nishi, Akinori, Ikarashi, Yasushi, Hattori, Tomohisa, Kase, Yoshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670863/
https://www.ncbi.nlm.nih.gov/pubmed/26681968
http://dx.doi.org/10.1155/2015/726471
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author Ueki, Toshiyuki
Mizoguchi, Kazushige
Yamaguchi, Takuji
Nishi, Akinori
Ikarashi, Yasushi
Hattori, Tomohisa
Kase, Yoshio
author_facet Ueki, Toshiyuki
Mizoguchi, Kazushige
Yamaguchi, Takuji
Nishi, Akinori
Ikarashi, Yasushi
Hattori, Tomohisa
Kase, Yoshio
author_sort Ueki, Toshiyuki
collection PubMed
description The traditional Japanese medicine yokukansan has an anxiolytic effect, which occurs after repeated administration. In this study, to investigate the underlying mechanisms, we examined the effects of repeated yokukansan administration on serotonin 1A (5-HT(1A)) receptor density and affinity and its expression at both mRNA and protein levels in the prefrontal cortex (PFC) of socially isolated mice. Moreover, we examined the effects of yokukansan on a 5-HT(1A) receptor-mediated behavioral response. Male mice were subjected to social isolation stress for 6 weeks and simultaneously treated with yokukansan. Thereafter, the density and affinity of 5-HT(1A) receptors were analyzed by a receptor-binding assay. Levels of 5-HT(1A) receptor protein and mRNA were also measured. Furthermore, (±)-8-hydroxy-2-(dipropylamino)tetralin hydrobromide (8-OH-DPAT; a 5-HT(1A) receptor agonist) was injected intraperitoneally, and rearing behavior was examined. Social isolation stress alone did not affect 5-HT(1A) receptor density or affinity. However, yokukansan significantly increased receptor density and decreased affinity concomitant with unchanged protein and mRNA levels. Yokukansan also enhanced the 8-OH-DPAT-induced decrease in rearing behavior. These results suggest that yokukansan increases 5-HT(1A) receptors in the PFC of socially isolated mice and enhances their function, which might underlie its anxiolytic effects.
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spelling pubmed-46708632015-12-17 Yokukansan Increases 5-HT(1A) Receptors in the Prefrontal Cortex and Enhances 5-HT(1A) Receptor Agonist-Induced Behavioral Responses in Socially Isolated Mice Ueki, Toshiyuki Mizoguchi, Kazushige Yamaguchi, Takuji Nishi, Akinori Ikarashi, Yasushi Hattori, Tomohisa Kase, Yoshio Evid Based Complement Alternat Med Research Article The traditional Japanese medicine yokukansan has an anxiolytic effect, which occurs after repeated administration. In this study, to investigate the underlying mechanisms, we examined the effects of repeated yokukansan administration on serotonin 1A (5-HT(1A)) receptor density and affinity and its expression at both mRNA and protein levels in the prefrontal cortex (PFC) of socially isolated mice. Moreover, we examined the effects of yokukansan on a 5-HT(1A) receptor-mediated behavioral response. Male mice were subjected to social isolation stress for 6 weeks and simultaneously treated with yokukansan. Thereafter, the density and affinity of 5-HT(1A) receptors were analyzed by a receptor-binding assay. Levels of 5-HT(1A) receptor protein and mRNA were also measured. Furthermore, (±)-8-hydroxy-2-(dipropylamino)tetralin hydrobromide (8-OH-DPAT; a 5-HT(1A) receptor agonist) was injected intraperitoneally, and rearing behavior was examined. Social isolation stress alone did not affect 5-HT(1A) receptor density or affinity. However, yokukansan significantly increased receptor density and decreased affinity concomitant with unchanged protein and mRNA levels. Yokukansan also enhanced the 8-OH-DPAT-induced decrease in rearing behavior. These results suggest that yokukansan increases 5-HT(1A) receptors in the PFC of socially isolated mice and enhances their function, which might underlie its anxiolytic effects. Hindawi Publishing Corporation 2015 2015-11-23 /pmc/articles/PMC4670863/ /pubmed/26681968 http://dx.doi.org/10.1155/2015/726471 Text en Copyright © 2015 Toshiyuki Ueki et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ueki, Toshiyuki
Mizoguchi, Kazushige
Yamaguchi, Takuji
Nishi, Akinori
Ikarashi, Yasushi
Hattori, Tomohisa
Kase, Yoshio
Yokukansan Increases 5-HT(1A) Receptors in the Prefrontal Cortex and Enhances 5-HT(1A) Receptor Agonist-Induced Behavioral Responses in Socially Isolated Mice
title Yokukansan Increases 5-HT(1A) Receptors in the Prefrontal Cortex and Enhances 5-HT(1A) Receptor Agonist-Induced Behavioral Responses in Socially Isolated Mice
title_full Yokukansan Increases 5-HT(1A) Receptors in the Prefrontal Cortex and Enhances 5-HT(1A) Receptor Agonist-Induced Behavioral Responses in Socially Isolated Mice
title_fullStr Yokukansan Increases 5-HT(1A) Receptors in the Prefrontal Cortex and Enhances 5-HT(1A) Receptor Agonist-Induced Behavioral Responses in Socially Isolated Mice
title_full_unstemmed Yokukansan Increases 5-HT(1A) Receptors in the Prefrontal Cortex and Enhances 5-HT(1A) Receptor Agonist-Induced Behavioral Responses in Socially Isolated Mice
title_short Yokukansan Increases 5-HT(1A) Receptors in the Prefrontal Cortex and Enhances 5-HT(1A) Receptor Agonist-Induced Behavioral Responses in Socially Isolated Mice
title_sort yokukansan increases 5-ht(1a) receptors in the prefrontal cortex and enhances 5-ht(1a) receptor agonist-induced behavioral responses in socially isolated mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670863/
https://www.ncbi.nlm.nih.gov/pubmed/26681968
http://dx.doi.org/10.1155/2015/726471
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