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Recombinant Passenger Proteins Can Be Conveniently Purified by One-Step Affinity Chromatography

Fusion tag is one of the best available tools to date for enhancement of the solubility or improvement of the expression level of recombinant proteins in Escherichia coli. Typically, two consecutive affinity purification steps are often necessitated for the purification of passenger proteins. As a f...

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Detalles Bibliográficos
Autores principales: Wang, Hua-zhen, Chu, Zhi-zhan, Chen, Chang-chao, Cao, Ao-cheng, Tong, Xin, Ouyang, Can-bin, Yuan, Qi-hang, Wang, Mi-nan, Wu, Zhong-kun, Wang, Hai-hong, Wang, Sheng-bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671538/
https://www.ncbi.nlm.nih.gov/pubmed/26641240
http://dx.doi.org/10.1371/journal.pone.0143598