Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress

Generation of multiple genomic alterations is currently a time consuming process. Here, a method was established that enables highly efficient and simultaneous deletion of multiple genes in Escherichia coli. A temperature sensitive plasmid containing arabinose inducible lambda Red recombineering gen...

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Detalles Bibliográficos
Autores principales: Jensen, Sheila I., Lennen, Rebecca M., Herrgård, Markus J., Nielsen, Alex T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672327/
https://www.ncbi.nlm.nih.gov/pubmed/26643270
http://dx.doi.org/10.1038/srep17874

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672327/
https://www.ncbi.nlm.nih.gov/pubmed/26643270
http://dx.doi.org/10.1038/srep17874

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