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Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress
Generation of multiple genomic alterations is currently a time consuming process. Here, a method was established that enables highly efficient and simultaneous deletion of multiple genes in Escherichia coli. A temperature sensitive plasmid containing arabinose inducible lambda Red recombineering gen...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672327/ https://www.ncbi.nlm.nih.gov/pubmed/26643270 http://dx.doi.org/10.1038/srep17874 |