Hair-bundle proteomes of avian and mammalian inner-ear utricles
Examination of multiple proteomics datasets within or between species increases the reliability of protein identification. We report here proteomes of inner-ear hair bundles from three species (chick, mouse, and rat), which were collected on LTQ or LTQ Velos ion-trap mass spectrometers; the constitu...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672683/ https://www.ncbi.nlm.nih.gov/pubmed/26645194 http://dx.doi.org/10.1038/sdata.2015.74 |
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author | Wilmarth, Phillip A. Krey, Jocelyn F. Shin, Jung-Bum Choi, Dongseok David, Larry L. Barr-Gillespie, Peter G. |
author_facet | Wilmarth, Phillip A. Krey, Jocelyn F. Shin, Jung-Bum Choi, Dongseok David, Larry L. Barr-Gillespie, Peter G. |
author_sort | Wilmarth, Phillip A. |
collection | PubMed |
description | Examination of multiple proteomics datasets within or between species increases the reliability of protein identification. We report here proteomes of inner-ear hair bundles from three species (chick, mouse, and rat), which were collected on LTQ or LTQ Velos ion-trap mass spectrometers; the constituent proteins were quantified using MS2 intensities, which are the summed intensities of all peptide fragmentation spectra matched to a protein. The data are available via ProteomeXchange with identifiers PXD002410 (chick LTQ), PXD002414 (chick Velos), PXD002415 (mouse Velos), and PXD002416 (rat LTQ). The two chick bundle datasets compared favourably to a third, already-described chick bundle dataset, which was quantified using MS1 peak intensities, the summed intensities of peptides identified by high-resolution mass spectrometry (PXD000104; updated analysis in PXD002445). The mouse bundle dataset described here was comparable to a different mouse bundle dataset quantified using MS1 intensities (PXD002167). These six datasets will be useful for identifying the core proteome of vestibular hair bundles. |
format | Online Article Text |
id | pubmed-4672683 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46726832015-12-10 Hair-bundle proteomes of avian and mammalian inner-ear utricles Wilmarth, Phillip A. Krey, Jocelyn F. Shin, Jung-Bum Choi, Dongseok David, Larry L. Barr-Gillespie, Peter G. Sci Data Data Descriptor Examination of multiple proteomics datasets within or between species increases the reliability of protein identification. We report here proteomes of inner-ear hair bundles from three species (chick, mouse, and rat), which were collected on LTQ or LTQ Velos ion-trap mass spectrometers; the constituent proteins were quantified using MS2 intensities, which are the summed intensities of all peptide fragmentation spectra matched to a protein. The data are available via ProteomeXchange with identifiers PXD002410 (chick LTQ), PXD002414 (chick Velos), PXD002415 (mouse Velos), and PXD002416 (rat LTQ). The two chick bundle datasets compared favourably to a third, already-described chick bundle dataset, which was quantified using MS1 peak intensities, the summed intensities of peptides identified by high-resolution mass spectrometry (PXD000104; updated analysis in PXD002445). The mouse bundle dataset described here was comparable to a different mouse bundle dataset quantified using MS1 intensities (PXD002167). These six datasets will be useful for identifying the core proteome of vestibular hair bundles. Nature Publishing Group 2015-12-08 /pmc/articles/PMC4672683/ /pubmed/26645194 http://dx.doi.org/10.1038/sdata.2015.74 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0 Metadata associated with this Data Descriptor is available at http://www.nature.com/sdata/ and is released under the CC0 waiver to maximize reuse. |
spellingShingle | Data Descriptor Wilmarth, Phillip A. Krey, Jocelyn F. Shin, Jung-Bum Choi, Dongseok David, Larry L. Barr-Gillespie, Peter G. Hair-bundle proteomes of avian and mammalian inner-ear utricles |
title | Hair-bundle proteomes of avian and mammalian inner-ear utricles |
title_full | Hair-bundle proteomes of avian and mammalian inner-ear utricles |
title_fullStr | Hair-bundle proteomes of avian and mammalian inner-ear utricles |
title_full_unstemmed | Hair-bundle proteomes of avian and mammalian inner-ear utricles |
title_short | Hair-bundle proteomes of avian and mammalian inner-ear utricles |
title_sort | hair-bundle proteomes of avian and mammalian inner-ear utricles |
topic | Data Descriptor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672683/ https://www.ncbi.nlm.nih.gov/pubmed/26645194 http://dx.doi.org/10.1038/sdata.2015.74 |
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