Hair-bundle proteomes of avian and mammalian inner-ear utricles

Examination of multiple proteomics datasets within or between species increases the reliability of protein identification. We report here proteomes of inner-ear hair bundles from three species (chick, mouse, and rat), which were collected on LTQ or LTQ Velos ion-trap mass spectrometers; the constitu...

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Autores principales: Wilmarth, Phillip A., Krey, Jocelyn F., Shin, Jung-Bum, Choi, Dongseok, David, Larry L., Barr-Gillespie, Peter G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672683/
https://www.ncbi.nlm.nih.gov/pubmed/26645194
http://dx.doi.org/10.1038/sdata.2015.74
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author Wilmarth, Phillip A.
Krey, Jocelyn F.
Shin, Jung-Bum
Choi, Dongseok
David, Larry L.
Barr-Gillespie, Peter G.
author_facet Wilmarth, Phillip A.
Krey, Jocelyn F.
Shin, Jung-Bum
Choi, Dongseok
David, Larry L.
Barr-Gillespie, Peter G.
author_sort Wilmarth, Phillip A.
collection PubMed
description Examination of multiple proteomics datasets within or between species increases the reliability of protein identification. We report here proteomes of inner-ear hair bundles from three species (chick, mouse, and rat), which were collected on LTQ or LTQ Velos ion-trap mass spectrometers; the constituent proteins were quantified using MS2 intensities, which are the summed intensities of all peptide fragmentation spectra matched to a protein. The data are available via ProteomeXchange with identifiers PXD002410 (chick LTQ), PXD002414 (chick Velos), PXD002415 (mouse Velos), and PXD002416 (rat LTQ). The two chick bundle datasets compared favourably to a third, already-described chick bundle dataset, which was quantified using MS1 peak intensities, the summed intensities of peptides identified by high-resolution mass spectrometry (PXD000104; updated analysis in PXD002445). The mouse bundle dataset described here was comparable to a different mouse bundle dataset quantified using MS1 intensities (PXD002167). These six datasets will be useful for identifying the core proteome of vestibular hair bundles.
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spelling pubmed-46726832015-12-10 Hair-bundle proteomes of avian and mammalian inner-ear utricles Wilmarth, Phillip A. Krey, Jocelyn F. Shin, Jung-Bum Choi, Dongseok David, Larry L. Barr-Gillespie, Peter G. Sci Data Data Descriptor Examination of multiple proteomics datasets within or between species increases the reliability of protein identification. We report here proteomes of inner-ear hair bundles from three species (chick, mouse, and rat), which were collected on LTQ or LTQ Velos ion-trap mass spectrometers; the constituent proteins were quantified using MS2 intensities, which are the summed intensities of all peptide fragmentation spectra matched to a protein. The data are available via ProteomeXchange with identifiers PXD002410 (chick LTQ), PXD002414 (chick Velos), PXD002415 (mouse Velos), and PXD002416 (rat LTQ). The two chick bundle datasets compared favourably to a third, already-described chick bundle dataset, which was quantified using MS1 peak intensities, the summed intensities of peptides identified by high-resolution mass spectrometry (PXD000104; updated analysis in PXD002445). The mouse bundle dataset described here was comparable to a different mouse bundle dataset quantified using MS1 intensities (PXD002167). These six datasets will be useful for identifying the core proteome of vestibular hair bundles. Nature Publishing Group 2015-12-08 /pmc/articles/PMC4672683/ /pubmed/26645194 http://dx.doi.org/10.1038/sdata.2015.74 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0 Metadata associated with this Data Descriptor is available at http://www.nature.com/sdata/ and is released under the CC0 waiver to maximize reuse.
spellingShingle Data Descriptor
Wilmarth, Phillip A.
Krey, Jocelyn F.
Shin, Jung-Bum
Choi, Dongseok
David, Larry L.
Barr-Gillespie, Peter G.
Hair-bundle proteomes of avian and mammalian inner-ear utricles
title Hair-bundle proteomes of avian and mammalian inner-ear utricles
title_full Hair-bundle proteomes of avian and mammalian inner-ear utricles
title_fullStr Hair-bundle proteomes of avian and mammalian inner-ear utricles
title_full_unstemmed Hair-bundle proteomes of avian and mammalian inner-ear utricles
title_short Hair-bundle proteomes of avian and mammalian inner-ear utricles
title_sort hair-bundle proteomes of avian and mammalian inner-ear utricles
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672683/
https://www.ncbi.nlm.nih.gov/pubmed/26645194
http://dx.doi.org/10.1038/sdata.2015.74
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