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Development of synthetic anti-cyclic citrullinated peptide antibody and its arthritogenic role
This study was undertaken to develop a novel anti-citrullinated peptide antibody (ACPA) and to investigate its arthritogenicity in a collagen-induced arthritis (CIA) model. The novel ACPA, 12G1, was developed by injecting cyclic citrullinated antigen in mice and subsequently hybridizing the B cells...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673436/ https://www.ncbi.nlm.nih.gov/pubmed/26682058 http://dx.doi.org/10.1038/cti.2015.24 |
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author | Kim, Youngkyun Lee, Jennifer Jung, Hyerin Yi, Hyoju Rim, Yeri Alice Jung, Seung Min Ju, Ji Hyeon |
author_facet | Kim, Youngkyun Lee, Jennifer Jung, Hyerin Yi, Hyoju Rim, Yeri Alice Jung, Seung Min Ju, Ji Hyeon |
author_sort | Kim, Youngkyun |
collection | PubMed |
description | This study was undertaken to develop a novel anti-citrullinated peptide antibody (ACPA) and to investigate its arthritogenicity in a collagen-induced arthritis (CIA) model. The novel ACPA, 12G1, was developed by injecting cyclic citrullinated antigen in mice and subsequently hybridizing the B cells producing citrullinated peptide-specific antibodies with a myeloma cell line. The arthritic joints of mice with CIA and collagen antibody-induced arthritis (CAIA) as well as interleukin-1 receptor antagonist (IL-1Ra) knockout (KO) mice were stained immunohistochemically using the 12G1 antibody. Confocal immunostaining was used to identify colocalization of 12G1 with various citrullinated proteins. 12G1 in the presence or absence of chelating beads was administered to CIA mice on days 21 and 28 after type II collagen (CII) immunization to investigate 12G1 arthritogenecity. 12G1 detected citrullinated proteins in the arthritic joints of all the experimental arthritis models used. Confocal immunostaining showed that 12G1 was colocalized with well-known citrullinated proteins, including vimentin, collagen, anti-immunoglobulin binding protein and fibronectin. Staining of citrullinated proteins using 12G1 was more diffuse in CIA mice compared with CAIA and IL-1Ra KO mice. 12G1 injection apparently acted as a booster of immunization in CIA mice in combination with a single CII immunization, with this effect being abolished when 12G1 was injected with chelating beads. The novel ACPA, 12G1, identified various citrullinated proteins in the arthritic joints of three experimental arthritis models. 12G1-treated mice developed arthritis following a single CII immunization, suggesting an arthritogenic potential for ACPA in CIA mice. |
format | Online Article Text |
id | pubmed-4673436 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46734362015-12-17 Development of synthetic anti-cyclic citrullinated peptide antibody and its arthritogenic role Kim, Youngkyun Lee, Jennifer Jung, Hyerin Yi, Hyoju Rim, Yeri Alice Jung, Seung Min Ju, Ji Hyeon Clin Transl Immunology Original Article This study was undertaken to develop a novel anti-citrullinated peptide antibody (ACPA) and to investigate its arthritogenicity in a collagen-induced arthritis (CIA) model. The novel ACPA, 12G1, was developed by injecting cyclic citrullinated antigen in mice and subsequently hybridizing the B cells producing citrullinated peptide-specific antibodies with a myeloma cell line. The arthritic joints of mice with CIA and collagen antibody-induced arthritis (CAIA) as well as interleukin-1 receptor antagonist (IL-1Ra) knockout (KO) mice were stained immunohistochemically using the 12G1 antibody. Confocal immunostaining was used to identify colocalization of 12G1 with various citrullinated proteins. 12G1 in the presence or absence of chelating beads was administered to CIA mice on days 21 and 28 after type II collagen (CII) immunization to investigate 12G1 arthritogenecity. 12G1 detected citrullinated proteins in the arthritic joints of all the experimental arthritis models used. Confocal immunostaining showed that 12G1 was colocalized with well-known citrullinated proteins, including vimentin, collagen, anti-immunoglobulin binding protein and fibronectin. Staining of citrullinated proteins using 12G1 was more diffuse in CIA mice compared with CAIA and IL-1Ra KO mice. 12G1 injection apparently acted as a booster of immunization in CIA mice in combination with a single CII immunization, with this effect being abolished when 12G1 was injected with chelating beads. The novel ACPA, 12G1, identified various citrullinated proteins in the arthritic joints of three experimental arthritis models. 12G1-treated mice developed arthritis following a single CII immunization, suggesting an arthritogenic potential for ACPA in CIA mice. Nature Publishing Group 2015-11-27 /pmc/articles/PMC4673436/ /pubmed/26682058 http://dx.doi.org/10.1038/cti.2015.24 Text en Copyright © 2015 Australasian Society for Immunology Inc. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Original Article Kim, Youngkyun Lee, Jennifer Jung, Hyerin Yi, Hyoju Rim, Yeri Alice Jung, Seung Min Ju, Ji Hyeon Development of synthetic anti-cyclic citrullinated peptide antibody and its arthritogenic role |
title | Development of synthetic anti-cyclic citrullinated peptide antibody and its arthritogenic role |
title_full | Development of synthetic anti-cyclic citrullinated peptide antibody and its arthritogenic role |
title_fullStr | Development of synthetic anti-cyclic citrullinated peptide antibody and its arthritogenic role |
title_full_unstemmed | Development of synthetic anti-cyclic citrullinated peptide antibody and its arthritogenic role |
title_short | Development of synthetic anti-cyclic citrullinated peptide antibody and its arthritogenic role |
title_sort | development of synthetic anti-cyclic citrullinated peptide antibody and its arthritogenic role |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673436/ https://www.ncbi.nlm.nih.gov/pubmed/26682058 http://dx.doi.org/10.1038/cti.2015.24 |
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