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Properties of targeted preamplification in DNA and cDNA quantification

Objective: Quantification of small molecule numbers often requires preamplification to generate enough copies for accurate downstream enumerations. Here, we studied experimental parameters in targeted preamplification and their effects on downstream quantitative real-time PCR (qPCR). Methods: To eva...

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Detalles Bibliográficos
Autores principales: Andersson, Daniel, Akrap, Nina, Svec, David, Godfrey, Tony E, Kubista, Mikael, Landberg, Göran, Ståhlberg, Anders
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Informa Healthcare 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673511/
https://www.ncbi.nlm.nih.gov/pubmed/26132215
http://dx.doi.org/10.1586/14737159.2015.1057124