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Exercise training restores the cardiac microRNA-1 and −214 levels regulating Ca(2+) handling after myocardial infarction

BACKGROUND: Impaired cardiomyocyte contractility and calcium handling are hallmarks of left ventricular contractile dysfunction. Exercise training has been used as a remarkable strategy in the treatment of heart disease. The microRNA-1, which targets sodium/calcium exchanger 1 (NCX), and microRNA-21...

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Autores principales: Melo, Stéphano Freitas Soares, Barauna, Valério Garrone, Neves, Vander José, Fernandes, Tiago, Lara, Lucienne da Silva, Mazzotti, Diego Robles, Oliveira, Edilamar Menezes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673865/
https://www.ncbi.nlm.nih.gov/pubmed/26646371
http://dx.doi.org/10.1186/s12872-015-0156-4
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author Melo, Stéphano Freitas Soares
Barauna, Valério Garrone
Neves, Vander José
Fernandes, Tiago
Lara, Lucienne da Silva
Mazzotti, Diego Robles
Oliveira, Edilamar Menezes
author_facet Melo, Stéphano Freitas Soares
Barauna, Valério Garrone
Neves, Vander José
Fernandes, Tiago
Lara, Lucienne da Silva
Mazzotti, Diego Robles
Oliveira, Edilamar Menezes
author_sort Melo, Stéphano Freitas Soares
collection PubMed
description BACKGROUND: Impaired cardiomyocyte contractility and calcium handling are hallmarks of left ventricular contractile dysfunction. Exercise training has been used as a remarkable strategy in the treatment of heart disease. The microRNA-1, which targets sodium/calcium exchanger 1 (NCX), and microRNA-214, which targets sarcoplasmic reticulum calcium ATPase-2a (Serca2a), are involved in cardiac function regulation. Thus, the aim of this study was to evaluate the effect of exercise training on cardiac microRNA-1 and −214 expression after myocardial infarction. METHODS: Wistar rats were randomized into four groups: sedentary sham (S-SHAM), sedentary infarction (S-INF), trained sham (T-SHAM), and trained infarction (T-INF). Exercise training consisted of 60 min/days, 5 days/week for 10 weeks with 3 % of body weight as overload beginning four weeks after myocardial infarction. RESULTS: MicroRNA-1 and −214 expressions were, respectively, decreased (52 %) and increased (54 %) in the S-INF compared to the S-SHAM, while exercise training normalized the expression of these microRNAs. The microRNA targets NCX and Serca-2a protein expression were, respectively, decreased (55 %) and increased (34 %) in the T-INF group compared to the S-INF group. CONCLUSIONS: These results suggest that exercise training restores microRNA-1 and −214 expression levels and prevents change in both NCX and Serca-2a protein and gene expressions. Altogether, our data suggest a molecular mechanism to restore ventricular function after exercise training in myocardial infarction rats.
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spelling pubmed-46738652015-12-10 Exercise training restores the cardiac microRNA-1 and −214 levels regulating Ca(2+) handling after myocardial infarction Melo, Stéphano Freitas Soares Barauna, Valério Garrone Neves, Vander José Fernandes, Tiago Lara, Lucienne da Silva Mazzotti, Diego Robles Oliveira, Edilamar Menezes BMC Cardiovasc Disord Research Article BACKGROUND: Impaired cardiomyocyte contractility and calcium handling are hallmarks of left ventricular contractile dysfunction. Exercise training has been used as a remarkable strategy in the treatment of heart disease. The microRNA-1, which targets sodium/calcium exchanger 1 (NCX), and microRNA-214, which targets sarcoplasmic reticulum calcium ATPase-2a (Serca2a), are involved in cardiac function regulation. Thus, the aim of this study was to evaluate the effect of exercise training on cardiac microRNA-1 and −214 expression after myocardial infarction. METHODS: Wistar rats were randomized into four groups: sedentary sham (S-SHAM), sedentary infarction (S-INF), trained sham (T-SHAM), and trained infarction (T-INF). Exercise training consisted of 60 min/days, 5 days/week for 10 weeks with 3 % of body weight as overload beginning four weeks after myocardial infarction. RESULTS: MicroRNA-1 and −214 expressions were, respectively, decreased (52 %) and increased (54 %) in the S-INF compared to the S-SHAM, while exercise training normalized the expression of these microRNAs. The microRNA targets NCX and Serca-2a protein expression were, respectively, decreased (55 %) and increased (34 %) in the T-INF group compared to the S-INF group. CONCLUSIONS: These results suggest that exercise training restores microRNA-1 and −214 expression levels and prevents change in both NCX and Serca-2a protein and gene expressions. Altogether, our data suggest a molecular mechanism to restore ventricular function after exercise training in myocardial infarction rats. BioMed Central 2015-12-09 /pmc/articles/PMC4673865/ /pubmed/26646371 http://dx.doi.org/10.1186/s12872-015-0156-4 Text en © Melo et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Melo, Stéphano Freitas Soares
Barauna, Valério Garrone
Neves, Vander José
Fernandes, Tiago
Lara, Lucienne da Silva
Mazzotti, Diego Robles
Oliveira, Edilamar Menezes
Exercise training restores the cardiac microRNA-1 and −214 levels regulating Ca(2+) handling after myocardial infarction
title Exercise training restores the cardiac microRNA-1 and −214 levels regulating Ca(2+) handling after myocardial infarction
title_full Exercise training restores the cardiac microRNA-1 and −214 levels regulating Ca(2+) handling after myocardial infarction
title_fullStr Exercise training restores the cardiac microRNA-1 and −214 levels regulating Ca(2+) handling after myocardial infarction
title_full_unstemmed Exercise training restores the cardiac microRNA-1 and −214 levels regulating Ca(2+) handling after myocardial infarction
title_short Exercise training restores the cardiac microRNA-1 and −214 levels regulating Ca(2+) handling after myocardial infarction
title_sort exercise training restores the cardiac microrna-1 and −214 levels regulating ca(2+) handling after myocardial infarction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673865/
https://www.ncbi.nlm.nih.gov/pubmed/26646371
http://dx.doi.org/10.1186/s12872-015-0156-4
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