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Novel Parallelized Electroporation by Electrostatic Manipulation of a Water-in-Oil Droplet as a Microreactor

Electroporation is the most widely used transfection method for delivery of cell-impermeable molecules into cells. We developed a novel gene transfection method, water-in-oil (W/O) droplet electroporation, using dielectric oil and an aqueous droplet containing mammalian cells and transgene DNA. When...

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Detalles Bibliográficos
Autores principales: Kurita, Hirofumi, Takahashi, Shota, Asada, Atsushi, Matsuo, Minako, Kishikawa, Kenta, Mizuno, Akira, Numano, Rika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674099/
https://www.ncbi.nlm.nih.gov/pubmed/26649904
http://dx.doi.org/10.1371/journal.pone.0144254