Cargando…
Novel Parallelized Electroporation by Electrostatic Manipulation of a Water-in-Oil Droplet as a Microreactor
Electroporation is the most widely used transfection method for delivery of cell-impermeable molecules into cells. We developed a novel gene transfection method, water-in-oil (W/O) droplet electroporation, using dielectric oil and an aqueous droplet containing mammalian cells and transgene DNA. When...
Autores principales: | Kurita, Hirofumi, Takahashi, Shota, Asada, Atsushi, Matsuo, Minako, Kishikawa, Kenta, Mizuno, Akira, Numano, Rika |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674099/ https://www.ncbi.nlm.nih.gov/pubmed/26649904 http://dx.doi.org/10.1371/journal.pone.0144254 |
Ejemplares similares
-
Fundamental study on a gene transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field
por: Kurita, Hirofumi, et al.
Publicado: (2016) -
Introduction of a plasmid and a protein into bovine and swine cells by water-in-oil droplet electroporation
por: ISHINO, Takeshi, et al.
Publicado: (2019) -
Mechanistic studies of gene delivery into mammalian cells by electrical short-circuiting via an aqueous droplet in dielectric oil
por: Kurita, Hirofumi, et al.
Publicado: (2020) -
Influence of Electroporation Medium on Delivery of Cell-Impermeable Small Molecules by Electrical Short-Circuiting via an Aqueous Droplet in Dielectric Oil: A Comparison of Different Fluorescent Tracers
por: Watanabe, Yuki, et al.
Publicado: (2022) -
Flow invariant droplet formation for stable parallel microreactors
por: Riche, Carson T., et al.
Publicado: (2016)