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Sequential fractionation and isolation of subcellular proteins from tissue or cultured cells
Many types of studies require the localization of a protein to, or isolation of enriched protein from a specific cellular compartment. Many protocols in the literature and from commercially available kits claim to yield pure cellular fractions. However, in our hands, the former often do not work eff...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678309/ https://www.ncbi.nlm.nih.gov/pubmed/26740924 http://dx.doi.org/10.1016/j.mex.2015.11.001 |