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Spontaneous Formation of RNA Strands, Peptidyl RNA, and Cofactors
How the biochemical machinery evolved from simple precursors is an open question. Here we show that ribonucleotides and amino acids condense to peptidyl RNAs in the absence of enzymes under conditions established for genetic copying. Untemplated formation of RNA strands that can encode genetic infor...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
WILEY-VCH Verlag
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678511/ https://www.ncbi.nlm.nih.gov/pubmed/26435376 http://dx.doi.org/10.1002/anie.201506593 |
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author | Jauker, Mario Griesser, Helmut Richert, Clemens |
author_facet | Jauker, Mario Griesser, Helmut Richert, Clemens |
author_sort | Jauker, Mario |
collection | PubMed |
description | How the biochemical machinery evolved from simple precursors is an open question. Here we show that ribonucleotides and amino acids condense to peptidyl RNAs in the absence of enzymes under conditions established for genetic copying. Untemplated formation of RNA strands that can encode genetic information, formation of peptidyl chains linked to RNA, and formation of the cofactors NAD(+), FAD, and ATP all occur under the same conditions. In the peptidyl RNAs, the peptide chains are phosphoramidate-linked to a ribonucleotide. Peptidyl RNAs with long peptide chains were selected from an initial pool when a lipophilic phase simulating the interior of membranes was offered, and free peptides were released upon acidification. Our results show that key molecules of genetics, catalysis, and metabolism can emerge under the same conditions, without a mineral surface, without an enzyme, and without the need for chemical pre-activation. |
format | Online Article Text |
id | pubmed-4678511 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | WILEY-VCH Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-46785112015-12-22 Spontaneous Formation of RNA Strands, Peptidyl RNA, and Cofactors Jauker, Mario Griesser, Helmut Richert, Clemens Angew Chem Int Ed Engl Communications How the biochemical machinery evolved from simple precursors is an open question. Here we show that ribonucleotides and amino acids condense to peptidyl RNAs in the absence of enzymes under conditions established for genetic copying. Untemplated formation of RNA strands that can encode genetic information, formation of peptidyl chains linked to RNA, and formation of the cofactors NAD(+), FAD, and ATP all occur under the same conditions. In the peptidyl RNAs, the peptide chains are phosphoramidate-linked to a ribonucleotide. Peptidyl RNAs with long peptide chains were selected from an initial pool when a lipophilic phase simulating the interior of membranes was offered, and free peptides were released upon acidification. Our results show that key molecules of genetics, catalysis, and metabolism can emerge under the same conditions, without a mineral surface, without an enzyme, and without the need for chemical pre-activation. WILEY-VCH Verlag 2015-11-23 2015-10-05 /pmc/articles/PMC4678511/ /pubmed/26435376 http://dx.doi.org/10.1002/anie.201506593 Text en © 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. https://creativecommons.org/licenses/by-nc/4.0/ © 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Communications Jauker, Mario Griesser, Helmut Richert, Clemens Spontaneous Formation of RNA Strands, Peptidyl RNA, and Cofactors |
title | Spontaneous Formation of RNA Strands, Peptidyl RNA, and Cofactors |
title_full | Spontaneous Formation of RNA Strands, Peptidyl RNA, and Cofactors |
title_fullStr | Spontaneous Formation of RNA Strands, Peptidyl RNA, and Cofactors |
title_full_unstemmed | Spontaneous Formation of RNA Strands, Peptidyl RNA, and Cofactors |
title_short | Spontaneous Formation of RNA Strands, Peptidyl RNA, and Cofactors |
title_sort | spontaneous formation of rna strands, peptidyl rna, and cofactors |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678511/ https://www.ncbi.nlm.nih.gov/pubmed/26435376 http://dx.doi.org/10.1002/anie.201506593 |
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