The dark side of ID8-Luc2: pitfalls for luciferase tagged murine models for ovarian cancer

Reliable mouse models are key in the discovery and development of novel anticancer treatments. Non-invasive monitoring techniques such as bioluminescence imaging (BLI) are useful tools to determine tumor engraftment and evaluate tumor growth. However, the development of ascites in ovarian cancer mou...

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Autores principales: Baert, Thaïs, Verschuere, Tina, Van Hoylandt, Anaïs, Gijsbers, Rik, Vergote, Ignace, Coosemans, An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Commentary
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678634/
https://www.ncbi.nlm.nih.gov/pubmed/26676113
http://dx.doi.org/10.1186/s40425-015-0102-0
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author Baert, Thaïs
Verschuere, Tina
Van Hoylandt, Anaïs
Gijsbers, Rik
Vergote, Ignace
Coosemans, An
author_facet Baert, Thaïs
Verschuere, Tina
Van Hoylandt, Anaïs
Gijsbers, Rik
Vergote, Ignace
Coosemans, An
author_sort Baert, Thaïs
collection PubMed
description Reliable mouse models are key in the discovery and development of novel anticancer treatments. Non-invasive monitoring techniques such as bioluminescence imaging (BLI) are useful tools to determine tumor engraftment and evaluate tumor growth. However, the development of ascites in ovarian cancer mouse models leads to possible difficulties. Ascites can interfere with the set-up of correct end points and can interfere with the evaluation of tumor volume using BLI. We provide optimized euthanasia criteria and in vivo data underlining the pitfalls of BLI.
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spelling pubmed-46786342015-12-16 The dark side of ID8-Luc2: pitfalls for luciferase tagged murine models for ovarian cancer Baert, Thaïs Verschuere, Tina Van Hoylandt, Anaïs Gijsbers, Rik Vergote, Ignace Coosemans, An J Immunother Cancer Commentary Reliable mouse models are key in the discovery and development of novel anticancer treatments. Non-invasive monitoring techniques such as bioluminescence imaging (BLI) are useful tools to determine tumor engraftment and evaluate tumor growth. However, the development of ascites in ovarian cancer mouse models leads to possible difficulties. Ascites can interfere with the set-up of correct end points and can interfere with the evaluation of tumor volume using BLI. We provide optimized euthanasia criteria and in vivo data underlining the pitfalls of BLI. BioMed Central 2015-12-15 /pmc/articles/PMC4678634/ /pubmed/26676113 http://dx.doi.org/10.1186/s40425-015-0102-0 Text en © Baert et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Commentary
Baert, Thaïs
Verschuere, Tina
Van Hoylandt, Anaïs
Gijsbers, Rik
Vergote, Ignace
Coosemans, An
The dark side of ID8-Luc2: pitfalls for luciferase tagged murine models for ovarian cancer
title The dark side of ID8-Luc2: pitfalls for luciferase tagged murine models for ovarian cancer
title_full The dark side of ID8-Luc2: pitfalls for luciferase tagged murine models for ovarian cancer
title_fullStr The dark side of ID8-Luc2: pitfalls for luciferase tagged murine models for ovarian cancer
title_full_unstemmed The dark side of ID8-Luc2: pitfalls for luciferase tagged murine models for ovarian cancer
title_short The dark side of ID8-Luc2: pitfalls for luciferase tagged murine models for ovarian cancer
title_sort dark side of id8-luc2: pitfalls for luciferase tagged murine models for ovarian cancer
topic Commentary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678634/
https://www.ncbi.nlm.nih.gov/pubmed/26676113
http://dx.doi.org/10.1186/s40425-015-0102-0
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