Site-specific replacement of the thymine methyl group by fluorine in thrombin binding aptamer significantly improves structural stability and anticoagulant activity

Here we report investigations, based on circular dichroism, nuclear magnetic resonance spectroscopy, molecular modelling, differential scanning calorimetry and prothrombin time assay, on analogues of the thrombin binding aptamer (TBA) in which individual thymidines were replaced by 5-fluoro-2′-deoxy...

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Detalles Bibliográficos
Autores principales: Virgilio, Antonella, Petraccone, Luigi, Vellecco, Valentina, Bucci, Mariarosaria, Varra, Michela, Irace, Carlo, Santamaria, Rita, Pepe, Antonietta, Mayol, Luciano, Esposito, Veronica, Galeone, Aldo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Chemical Biology and Nucleic Acid Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678827/
https://www.ncbi.nlm.nih.gov/pubmed/26582916
http://dx.doi.org/10.1093/nar/gkv1224

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678827/
https://www.ncbi.nlm.nih.gov/pubmed/26582916
http://dx.doi.org/10.1093/nar/gkv1224

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