CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity

CRISPR/Cas9 has emerged as a versatile genome-engineering tool that relies on a single guide RNA (sgRNA) and the Cas9 enzyme for genome editing. Simple, fast and economical methods to generate sgRNAs have made targeted mutagenesis routine in cultured cells, mice, zebrafish and other model systems. P...

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Detalles Bibliográficos
Autores principales: Carrington, Blake, Varshney, Gaurav K., Burgess, Shawn M., Sood, Raman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678847/
https://www.ncbi.nlm.nih.gov/pubmed/26253739
http://dx.doi.org/10.1093/nar/gkv802

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678847/
https://www.ncbi.nlm.nih.gov/pubmed/26253739
http://dx.doi.org/10.1093/nar/gkv802

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