Development of a mono-promoter-driven CRISPR/Cas9 system in mammalian cells

The CRISPR/Cas9 system has been used for spatio-temporal gene modification through the ubiquitous expression of gRNA by an RNA polymerase III promoter and the controlled expression of Cas9 using a tissue-specific or inducible promoter. However, unexpected gene disruptions indicate the necessity of a...

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Detalles Bibliográficos
Autores principales: Yoshioka, Shin, Fujii, Wataru, Ogawa, Tetsuhiro, Sugiura, Koji, Naito, Kunihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4680873/
https://www.ncbi.nlm.nih.gov/pubmed/26669567
http://dx.doi.org/10.1038/srep18341

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4680873/
https://www.ncbi.nlm.nih.gov/pubmed/26669567
http://dx.doi.org/10.1038/srep18341

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