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Leukotriene C(4) is the major trigger of stress-induced oxidative DNA damage
Endoplasmic reticulum (ER) stress and major chemotherapeutic agents damage DNA by generating reactive oxygen species (ROS). Here we show that ER stress and chemotherapy induce leukotriene C(4) (LTC(4)) biosynthesis by transcriptionally upregulating and activating the enzyme microsomal glutathione-S-...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4682057/ https://www.ncbi.nlm.nih.gov/pubmed/26656251 http://dx.doi.org/10.1038/ncomms10112 |
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author | Dvash, Efrat Har-Tal, Michal Barak, Sara Meir, Ofir Rubinstein, Menachem |
author_facet | Dvash, Efrat Har-Tal, Michal Barak, Sara Meir, Ofir Rubinstein, Menachem |
author_sort | Dvash, Efrat |
collection | PubMed |
description | Endoplasmic reticulum (ER) stress and major chemotherapeutic agents damage DNA by generating reactive oxygen species (ROS). Here we show that ER stress and chemotherapy induce leukotriene C(4) (LTC(4)) biosynthesis by transcriptionally upregulating and activating the enzyme microsomal glutathione-S-transferase 2 (MGST2) in cells of non-haematopoietic lineage. ER stress and chemotherapy also trigger nuclear translocation of the two LTC(4) receptors. Acting in an intracrine manner, LTC(4) then elicits nuclear translocation of NADPH oxidase 4 (NOX4), ROS accumulation and oxidative DNA damage. Mgst2 deficiency, RNAi and LTC(4) receptor antagonists abolish ER stress- and chemotherapy-induced ROS and oxidative DNA damage in vitro and in mouse kidneys. Cell death and mouse morbidity are also significantly attenuated. Hence, MGST2-generated LTC(4) is a major mediator of ER stress- and chemotherapy-triggered oxidative stress and oxidative DNA damage. LTC(4) inhibitors, commonly used for asthma, could find broad clinical use in major human pathologies associated with ER stress-activated NOX4. |
format | Online Article Text |
id | pubmed-4682057 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46820572015-12-29 Leukotriene C(4) is the major trigger of stress-induced oxidative DNA damage Dvash, Efrat Har-Tal, Michal Barak, Sara Meir, Ofir Rubinstein, Menachem Nat Commun Article Endoplasmic reticulum (ER) stress and major chemotherapeutic agents damage DNA by generating reactive oxygen species (ROS). Here we show that ER stress and chemotherapy induce leukotriene C(4) (LTC(4)) biosynthesis by transcriptionally upregulating and activating the enzyme microsomal glutathione-S-transferase 2 (MGST2) in cells of non-haematopoietic lineage. ER stress and chemotherapy also trigger nuclear translocation of the two LTC(4) receptors. Acting in an intracrine manner, LTC(4) then elicits nuclear translocation of NADPH oxidase 4 (NOX4), ROS accumulation and oxidative DNA damage. Mgst2 deficiency, RNAi and LTC(4) receptor antagonists abolish ER stress- and chemotherapy-induced ROS and oxidative DNA damage in vitro and in mouse kidneys. Cell death and mouse morbidity are also significantly attenuated. Hence, MGST2-generated LTC(4) is a major mediator of ER stress- and chemotherapy-triggered oxidative stress and oxidative DNA damage. LTC(4) inhibitors, commonly used for asthma, could find broad clinical use in major human pathologies associated with ER stress-activated NOX4. Nature Publishing Group 2015-12-11 /pmc/articles/PMC4682057/ /pubmed/26656251 http://dx.doi.org/10.1038/ncomms10112 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Dvash, Efrat Har-Tal, Michal Barak, Sara Meir, Ofir Rubinstein, Menachem Leukotriene C(4) is the major trigger of stress-induced oxidative DNA damage |
title | Leukotriene C(4) is the major trigger of stress-induced oxidative DNA damage |
title_full | Leukotriene C(4) is the major trigger of stress-induced oxidative DNA damage |
title_fullStr | Leukotriene C(4) is the major trigger of stress-induced oxidative DNA damage |
title_full_unstemmed | Leukotriene C(4) is the major trigger of stress-induced oxidative DNA damage |
title_short | Leukotriene C(4) is the major trigger of stress-induced oxidative DNA damage |
title_sort | leukotriene c(4) is the major trigger of stress-induced oxidative dna damage |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4682057/ https://www.ncbi.nlm.nih.gov/pubmed/26656251 http://dx.doi.org/10.1038/ncomms10112 |
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