Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase

[Image: see text] A single mutation within a flavoprotein is capable of switching the catalytic activity of a dehalogenase into a nitroreductase. This change in function correlates with a destabilization of the one-electron-reduced flavin semiquinone that is differentially expressed in the nitro-FMN...

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Autores principales: Mukherjee, Arnab, Rokita, Steven E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2015
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684082/
https://www.ncbi.nlm.nih.gov/pubmed/26616824
http://dx.doi.org/10.1021/jacs.5b07540
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author Mukherjee, Arnab
Rokita, Steven E.
author_facet Mukherjee, Arnab
Rokita, Steven E.
author_sort Mukherjee, Arnab
collection PubMed
description [Image: see text] A single mutation within a flavoprotein is capable of switching the catalytic activity of a dehalogenase into a nitroreductase. This change in function correlates with a destabilization of the one-electron-reduced flavin semiquinone that is differentially expressed in the nitro-FMN reductase superfamily during redox cycling. The diversity of function within such a superfamily therefore has the potential to arise from rapid evolution, and its members should provide a convenient basis for developing new catalysts with an altered specificity of choice.
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spelling pubmed-46840822016-11-30 Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase Mukherjee, Arnab Rokita, Steven E. J Am Chem Soc [Image: see text] A single mutation within a flavoprotein is capable of switching the catalytic activity of a dehalogenase into a nitroreductase. This change in function correlates with a destabilization of the one-electron-reduced flavin semiquinone that is differentially expressed in the nitro-FMN reductase superfamily during redox cycling. The diversity of function within such a superfamily therefore has the potential to arise from rapid evolution, and its members should provide a convenient basis for developing new catalysts with an altered specificity of choice. American Chemical Society 2015-11-30 2015-12-16 /pmc/articles/PMC4684082/ /pubmed/26616824 http://dx.doi.org/10.1021/jacs.5b07540 Text en Copyright © 2015 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Mukherjee, Arnab
Rokita, Steven E.
Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase
title Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase
title_full Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase
title_fullStr Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase
title_full_unstemmed Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase
title_short Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase
title_sort single amino acid switch between a flavin-dependent dehalogenase and nitroreductase
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684082/
https://www.ncbi.nlm.nih.gov/pubmed/26616824
http://dx.doi.org/10.1021/jacs.5b07540
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