Identification of the nucleophile catalytic residue of GH51 α-l-arabinofuranosidase from Pleurotus ostreatus

In this study, the recombinant α-l-arabinofuranosidase from the fungus Pleurotus ostreatus (rPoAbf) was subjected to site-directed mutagenesis in order to identify the catalytic nucleophile residue. Based on bioinformatics and homology modelling analyses, E449 was revealed to be the potential nucleo...

Descripción completa

Detalles Bibliográficos
Autores principales: Amore, Antonella, Iadonisi, Alfonso, Vincent, Florence, Faraco, Vincenza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686458/
https://www.ncbi.nlm.nih.gov/pubmed/26690659
http://dx.doi.org/10.1186/s13568-015-0164-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686458/
https://www.ncbi.nlm.nih.gov/pubmed/26690659
http://dx.doi.org/10.1186/s13568-015-0164-x

Ejemplares similares