Addressing Bias in Small RNA Library Preparation for Sequencing: A New Protocol Recovers MicroRNAs that Evade Capture by Current Methods

Recent advances in sequencing technology have helped unveil the unexpected complexity and diversity of small RNAs. A critical step in small RNA library preparation for sequencing is the ligation of adapter sequences to both the 5′ and 3′ ends of small RNAs. Studies have shown that adapter ligation i...

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Detalles Bibliográficos
Autores principales: Baran-Gale, Jeanette, Kurtz, C. Lisa, Erdos, Michael R., Sison, Christina, Young, Alice, Fannin, Emily E., Chines, Peter S., Sethupathy, Praveen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686641/
https://www.ncbi.nlm.nih.gov/pubmed/26734062
http://dx.doi.org/10.3389/fgene.2015.00352

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686641/
https://www.ncbi.nlm.nih.gov/pubmed/26734062
http://dx.doi.org/10.3389/fgene.2015.00352

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