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Addressing Bias in Small RNA Library Preparation for Sequencing: A New Protocol Recovers MicroRNAs that Evade Capture by Current Methods
Recent advances in sequencing technology have helped unveil the unexpected complexity and diversity of small RNAs. A critical step in small RNA library preparation for sequencing is the ligation of adapter sequences to both the 5′ and 3′ ends of small RNAs. Studies have shown that adapter ligation i...
Autores principales: | Baran-Gale, Jeanette, Kurtz, C. Lisa, Erdos, Michael R., Sison, Christina, Young, Alice, Fannin, Emily E., Chines, Peter S., Sethupathy, Praveen |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686641/ https://www.ncbi.nlm.nih.gov/pubmed/26734062 http://dx.doi.org/10.3389/fgene.2015.00352 |
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