Cargando…

Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics

Unculturable bacterial communities provide a rich source of biocatalysts, but their experimental discovery by functional metagenomics is difficult, because the odds are stacked against the experimentor. Here we demonstrate functional screening of a million-membered metagenomic library in microfluidi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Colin, Pierre-Yves, Kintses, Balint, Gielen, Fabrice, Miton, Charlotte M., Fischer, Gerhard, Mohamed, Mark F., Hyvönen, Marko, Morgavi, Diego P., Janssen, Dick B, Hollfelder, Florian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686663/ https://www.ncbi.nlm.nih.gov/pubmed/26639611 http://dx.doi.org/10.1038/ncomms10008

Ejemplares similares

Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments
por: Gantz, Maximilian, et al.
Publicado: (2023)

Picodroplet partitioned whole genome amplification of low biomass samples preserves genomic diversity for metagenomic analysis
por: Hammond, Maria, et al.
Publicado: (2016)

Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput
por: Holstein, Josephin M., et al.
Publicado: (2021)

A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution
por: Alex Wong, Che Fai, et al.
Publicado: (2021)

Customizing droplet contents and dynamic ranges via integrated programmable picodroplet assembler
por: Zhang, Pengfei, et al.
Publicado: (2019)

“NAD‐display”: Ultrahigh‐Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry
por: Lindenburg, Laurens, et al.
Publicado: (2021)

High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform
por: Neun, Stefanie, et al.
Publicado: (2022)

Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins
por: Lindenburg, Laurens, et al.
Publicado: (2020)

Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase
por: Schnettler, J. David, et al.
Publicado: (2022)

One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution
por: Zinchenko, Anastasia, et al.
Publicado: (2014)

Ultrahigh‐Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay
por: Klaus, Miriam, et al.
Publicado: (2021)

Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets
por: Ladeveze, Simon, et al.
Publicado: (2023)

Strain-level profiling with picodroplet microfluidic cultivation reveals host-specific adaption of honeybee gut symbionts
por: Meng, Yujie, et al.
Publicado: (2022)

Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets
por: Gielen, Fabrice, et al.
Publicado: (2017)

Ultrahigh-Throughput Improvement and Discovery of Enzymes Using Droplet-Based Microfluidic Screening
por: Autour, Alexis, et al.
Publicado: (2017)

Advances in Ultrahigh Throughput Hit Discovery with Tandem Mass Spectrometry Encoded Libraries
por: Mata, J. Miguel, et al.
Publicado: (2023)

Discovery of carbamate degrading enzymes by functional metagenomics
por: Ufarté, Lisa, et al.
Publicado: (2017)

Development of novel noninvasive prenatal testing protocol for whole autosomal recessive disease using picodroplet digital PCR
por: Chang, Mun Young, et al.
Publicado: (2016)

Gene Capture Coupled to High-Throughput Sequencing as a Strategy for Targeted Metagenome Exploration
por: Denonfoux, Jérémie, et al.
Publicado: (2013)

Ultrahigh-throughput functional profiling of microbiota communities
por: Terekhov, Stanislav S., et al.
Publicado: (2018)

Highly Promiscuous Oxidases Discovered in the Bovine Rumen Microbiome
por: Ufarté, Lisa, et al.
Publicado: (2018)

Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity
por: Kaltenbach, Miriam, et al.
Publicado: (2016)

Insights into the evolution of enzyme substrate promiscuity after the discovery of (βα)(8) isomerase evolutionary intermediates from a diverse metagenome
por: Noda-García, Lianet, et al.
Publicado: (2015)

Discovery of novel geranylgeranyl reductases and characterization of their substrate promiscuity
por: Meadows, Corey W., et al.
Publicado: (2018)

Activity artifacts in drug discovery and different facets of compound promiscuity
por: Bajorath, Jürgen
Publicado: (2014)

Multiplex Ultrasensitive Genotyping of Patients with Non-Small Cell Lung Cancer for Epidermal Growth Factor Receptor (EGFR) Mutations by Means of Picodroplet Digital PCR
por: Watanabe, Masaru, et al.
Publicado: (2017)

Senescence chips for ultrahigh‐throughput isolation and removal of senescent cells
por: Chen, Yuchao, et al.
Publicado: (2018)

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
por: Demaree, Benjamin, et al.
Publicado: (2018)

High-Throughput Screening of the Thermoelastic Properties of Ultrahigh-Temperature Ceramics
por: Nath, Pinku, et al.
Publicado: (2021)

Ultrahigh‐Throughput Screening of an Artificial Metalloenzyme using Double Emulsions
por: Vallapurackal, Jaicy, et al.
Publicado: (2022)

Multidimensional Protein Solubility Optimization with an Ultrahigh-Throughput Microfluidic Platform
por: Erkamp, Nadia A., et al.
Publicado: (2023)

Specificity Effects of Amino Acid Substitutions in Promiscuous Hydrolases: Context‐Dependence of Catalytic Residue Contributions to Local Fitness Landscapes in Nearby Sequence Space
por: Bayer, Christopher D., et al.
Publicado: (2017)

Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture
por: Knyphausen, Philipp, et al.
Publicado: (2023)

Acoustic Mist Ionization Mass Spectrometry for Ultrahigh-Throughput Metabolomics Screening
por: Smith, Matthew J., et al.
Publicado: (2021)

Promiscuity mapping of the S100 protein family using a high-throughput holdup assay
por: Simon, Márton A., et al.
Publicado: (2022)

MetaEuk—sensitive, high-throughput gene discovery, and annotation for large-scale eukaryotic metagenomics
por: Levy Karin, Eli, et al.
Publicado: (2020)

An Improved Single Cell Ultrahigh Throughput Screening Method Based on In Vitro Compartmentalization
por: Ma, Fuqiang, et al.
Publicado: (2014)

Ultrahigh-Throughput Production of Monodisperse and Multifunctional Janus Microparticles Using in-Air Microfluidics
por: Kamperman, Tom, et al.
Publicado: (2018)

Abseq: Ultrahigh-throughput single cell protein profiling with droplet microfluidic barcoding
por: Shahi, Payam, et al.
Publicado: (2017)

20 years of crystal hits: progress and promise in ultrahigh-throughput crystallization screening
por: Lynch, Miranda L., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_0m1qmb6dng0c1e5v7ofqd85jjh