Target engagement and drug residence time can be observed in living cells with BRET

The therapeutic action of drugs is predicated on their physical engagement with cellular targets. Here we describe a broadly applicable method using bioluminescence resonance energy transfer (BRET) to reveal the binding characteristics of a drug with selected targets within intact cells. Cell-permea...

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Detalles Bibliográficos
Autores principales: Robers, Matthew B., Dart, Melanie L., Woodroofe, Carolyn C., Zimprich, Chad A., Kirkland, Thomas A., Machleidt, Thomas, Kupcho, Kevin R., Levin, Sergiy, Hartnett, James R., Zimmerman, Kristopher, Niles, Andrew L., Ohana, Rachel Friedman, Daniels, Danette L., Slater, Michael, Wood, Monika G., Cong, Mei, Cheng, Yi-Qiang, Wood, Keith V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686764/
https://www.ncbi.nlm.nih.gov/pubmed/26631872
http://dx.doi.org/10.1038/ncomms10091

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686764/
https://www.ncbi.nlm.nih.gov/pubmed/26631872
http://dx.doi.org/10.1038/ncomms10091

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