PAM multiplicity marks genomic target sites as inhibitory to CRISPR-Cas9 editing

In CRISPR-Cas9 genome editing, the underlying principles for selecting guide RNA (gRNA) sequences that would ensure for efficient target site modification remain poorly understood. Here we show that target sites harbouring multiple protospacer adjacent motifs (PAMs) are refractory to Cas9-mediated r...

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Detalles Bibliográficos
Autores principales: Malina, Abba, Cameron, Christopher J. F., Robert, Francis, Blanchette, Mathieu, Dostie, Josée, Pelletier, Jerry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686818/
https://www.ncbi.nlm.nih.gov/pubmed/26644285
http://dx.doi.org/10.1038/ncomms10124

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686818/
https://www.ncbi.nlm.nih.gov/pubmed/26644285
http://dx.doi.org/10.1038/ncomms10124

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