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A conformational RNA zipper promotes intron ejection during non‐conventional XBP1 mRNA splicing
The kinase/endonuclease IRE1 is the most conserved signal transducer of the unfolded protein response (UPR), an intracellular signaling network that monitors and regulates the protein folding capacity of the endoplasmic reticulum (ER). Upon sensing protein folding perturbations in the ER, IRE1 initi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687415/ https://www.ncbi.nlm.nih.gov/pubmed/26483401 http://dx.doi.org/10.15252/embr.201540955 |
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author | Peschek, Jirka Acosta‐Alvear, Diego Mendez, Aaron S Walter, Peter |
author_facet | Peschek, Jirka Acosta‐Alvear, Diego Mendez, Aaron S Walter, Peter |
author_sort | Peschek, Jirka |
collection | PubMed |
description | The kinase/endonuclease IRE1 is the most conserved signal transducer of the unfolded protein response (UPR), an intracellular signaling network that monitors and regulates the protein folding capacity of the endoplasmic reticulum (ER). Upon sensing protein folding perturbations in the ER, IRE1 initiates the unconventional splicing of XBP1 mRNA culminating in the production of the transcription factor XBP1s, which expands the ER's protein folding capacity. We show that an RNA‐intrinsic conformational change causes the intron of XBP1 mRNA to be ejected and the exons to zipper up into an extended stem, juxtaposing the RNA ends for ligation. These conformational rearrangements are important for XBP1 mRNA splicing in vivo. The features that point to such active participation of XBP1 mRNA in the splicing reaction are highly conserved throughout metazoan evolution, supporting their importance in orchestrating XBP1 mRNA processing with efficiency and fidelity. |
format | Online Article Text |
id | pubmed-4687415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46874152015-12-30 A conformational RNA zipper promotes intron ejection during non‐conventional XBP1 mRNA splicing Peschek, Jirka Acosta‐Alvear, Diego Mendez, Aaron S Walter, Peter EMBO Rep Articles The kinase/endonuclease IRE1 is the most conserved signal transducer of the unfolded protein response (UPR), an intracellular signaling network that monitors and regulates the protein folding capacity of the endoplasmic reticulum (ER). Upon sensing protein folding perturbations in the ER, IRE1 initiates the unconventional splicing of XBP1 mRNA culminating in the production of the transcription factor XBP1s, which expands the ER's protein folding capacity. We show that an RNA‐intrinsic conformational change causes the intron of XBP1 mRNA to be ejected and the exons to zipper up into an extended stem, juxtaposing the RNA ends for ligation. These conformational rearrangements are important for XBP1 mRNA splicing in vivo. The features that point to such active participation of XBP1 mRNA in the splicing reaction are highly conserved throughout metazoan evolution, supporting their importance in orchestrating XBP1 mRNA processing with efficiency and fidelity. John Wiley and Sons Inc. 2015-10-19 2015-12 /pmc/articles/PMC4687415/ /pubmed/26483401 http://dx.doi.org/10.15252/embr.201540955 Text en © 2015 The Authors. Published under the terms of the CC BY NC ND 4.0 license This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Articles Peschek, Jirka Acosta‐Alvear, Diego Mendez, Aaron S Walter, Peter A conformational RNA zipper promotes intron ejection during non‐conventional XBP1 mRNA splicing |
title | A conformational RNA zipper promotes intron ejection during non‐conventional XBP1
mRNA splicing |
title_full | A conformational RNA zipper promotes intron ejection during non‐conventional XBP1
mRNA splicing |
title_fullStr | A conformational RNA zipper promotes intron ejection during non‐conventional XBP1
mRNA splicing |
title_full_unstemmed | A conformational RNA zipper promotes intron ejection during non‐conventional XBP1
mRNA splicing |
title_short | A conformational RNA zipper promotes intron ejection during non‐conventional XBP1
mRNA splicing |
title_sort | conformational rna zipper promotes intron ejection during non‐conventional xbp1
mrna splicing |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687415/ https://www.ncbi.nlm.nih.gov/pubmed/26483401 http://dx.doi.org/10.15252/embr.201540955 |
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