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Glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin ArtinM

This article contains data related to the researc.h article entitled “Yeast-derived ArtinM shares structure, carbohydrate recognition, and biological effects with native ArtinM” by Cecílio et al. (2015) [1]. ArtinM, a D-mannose-binding lectin isolated from the seeds of Artocarpus heterophyllus, exer...

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Autores principales: Liu, Y., Cecílio, N.T., Carvalho, F.C., Roque-Barreira, M.C., Feizi, T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689119/
https://www.ncbi.nlm.nih.gov/pubmed/26793748
http://dx.doi.org/10.1016/j.dib.2015.11.014
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author Liu, Y.
Cecílio, N.T.
Carvalho, F.C.
Roque-Barreira, M.C.
Feizi, T.
author_facet Liu, Y.
Cecílio, N.T.
Carvalho, F.C.
Roque-Barreira, M.C.
Feizi, T.
author_sort Liu, Y.
collection PubMed
description This article contains data related to the researc.h article entitled “Yeast-derived ArtinM shares structure, carbohydrate recognition, and biological effects with native ArtinM” by Cecílio et al. (2015) [1]. ArtinM, a D-mannose-binding lectin isolated from the seeds of Artocarpus heterophyllus, exerts immunomodulatory and regenerative activities through its Carbohydrate Recognition Domain (CRD) (Souza et al., 2013; Mariano et al., 2014 [2], [3]). The limited availability of the native lectin (n-ArtinM) led us to characterize a recombinant form of the protein, obtained by expression in Saccharomyces cerevisiae (y-ArtinM). We compared the carbohydrate-binding specificities of y-ArtinM and n-ArtinM by analyzing the binding of biotinylated preparations of the two lectin forms using a neoglycolipid (NGL)-based glycan microarray. Data showed that y-ArtinM mirrored the specificity exhibited by n-ArtinM.
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spelling pubmed-46891192016-01-20 Glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin ArtinM Liu, Y. Cecílio, N.T. Carvalho, F.C. Roque-Barreira, M.C. Feizi, T. Data Brief Data Article This article contains data related to the researc.h article entitled “Yeast-derived ArtinM shares structure, carbohydrate recognition, and biological effects with native ArtinM” by Cecílio et al. (2015) [1]. ArtinM, a D-mannose-binding lectin isolated from the seeds of Artocarpus heterophyllus, exerts immunomodulatory and regenerative activities through its Carbohydrate Recognition Domain (CRD) (Souza et al., 2013; Mariano et al., 2014 [2], [3]). The limited availability of the native lectin (n-ArtinM) led us to characterize a recombinant form of the protein, obtained by expression in Saccharomyces cerevisiae (y-ArtinM). We compared the carbohydrate-binding specificities of y-ArtinM and n-ArtinM by analyzing the binding of biotinylated preparations of the two lectin forms using a neoglycolipid (NGL)-based glycan microarray. Data showed that y-ArtinM mirrored the specificity exhibited by n-ArtinM. Elsevier 2015-11-18 /pmc/articles/PMC4689119/ /pubmed/26793748 http://dx.doi.org/10.1016/j.dib.2015.11.014 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Liu, Y.
Cecílio, N.T.
Carvalho, F.C.
Roque-Barreira, M.C.
Feizi, T.
Glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin ArtinM
title Glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin ArtinM
title_full Glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin ArtinM
title_fullStr Glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin ArtinM
title_full_unstemmed Glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin ArtinM
title_short Glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin ArtinM
title_sort glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin artinm
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689119/
https://www.ncbi.nlm.nih.gov/pubmed/26793748
http://dx.doi.org/10.1016/j.dib.2015.11.014
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