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MC-LR Exposure Leads to Subfertility of Female Mice and Induces Oxidative Stress in Granulosa Cells

Health risk of human exposure to microcystin-leucine arginine (MC-LR) has aroused more and more attention over the past few decades. In the present study, MC-LR was orally administered to female mice at 0, 1, 10 and 40 μg/L for three and six months. We found that chronic exposure to MC-LR at environ...

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Detalles Bibliográficos
Autores principales: Wu, Jiang, Yuan, Mingming, Song, Yuefeng, Sun, Feng, Han, Xiaodong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690122/
https://www.ncbi.nlm.nih.gov/pubmed/26633508
http://dx.doi.org/10.3390/toxins7124872
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author Wu, Jiang
Yuan, Mingming
Song, Yuefeng
Sun, Feng
Han, Xiaodong
author_facet Wu, Jiang
Yuan, Mingming
Song, Yuefeng
Sun, Feng
Han, Xiaodong
author_sort Wu, Jiang
collection PubMed
description Health risk of human exposure to microcystin-leucine arginine (MC-LR) has aroused more and more attention over the past few decades. In the present study, MC-LR was orally administered to female mice at 0, 1, 10 and 40 μg/L for three and six months. We found that chronic exposure to MC-LR at environmental levels could stimulate follicle atresia and lead to decreased developmental follicles, accompanied by a reduction of gonadosomatic index (GSI). In line with the irregular gonadal hormone level and estrus cycles, subfertility of female mice was also confirmed by analyzing numbers of litters and pups. The in vitro study suggested that granulosa cells could uptake MC-LR and should be the target of the toxicant. Oxidative stress in granulose cells induced by MC-LR promoted follicle atresia and eventually leads to female subfertility.
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spelling pubmed-46901222015-12-30 MC-LR Exposure Leads to Subfertility of Female Mice and Induces Oxidative Stress in Granulosa Cells Wu, Jiang Yuan, Mingming Song, Yuefeng Sun, Feng Han, Xiaodong Toxins (Basel) Article Health risk of human exposure to microcystin-leucine arginine (MC-LR) has aroused more and more attention over the past few decades. In the present study, MC-LR was orally administered to female mice at 0, 1, 10 and 40 μg/L for three and six months. We found that chronic exposure to MC-LR at environmental levels could stimulate follicle atresia and lead to decreased developmental follicles, accompanied by a reduction of gonadosomatic index (GSI). In line with the irregular gonadal hormone level and estrus cycles, subfertility of female mice was also confirmed by analyzing numbers of litters and pups. The in vitro study suggested that granulosa cells could uptake MC-LR and should be the target of the toxicant. Oxidative stress in granulose cells induced by MC-LR promoted follicle atresia and eventually leads to female subfertility. MDPI 2015-12-02 /pmc/articles/PMC4690122/ /pubmed/26633508 http://dx.doi.org/10.3390/toxins7124872 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Jiang
Yuan, Mingming
Song, Yuefeng
Sun, Feng
Han, Xiaodong
MC-LR Exposure Leads to Subfertility of Female Mice and Induces Oxidative Stress in Granulosa Cells
title MC-LR Exposure Leads to Subfertility of Female Mice and Induces Oxidative Stress in Granulosa Cells
title_full MC-LR Exposure Leads to Subfertility of Female Mice and Induces Oxidative Stress in Granulosa Cells
title_fullStr MC-LR Exposure Leads to Subfertility of Female Mice and Induces Oxidative Stress in Granulosa Cells
title_full_unstemmed MC-LR Exposure Leads to Subfertility of Female Mice and Induces Oxidative Stress in Granulosa Cells
title_short MC-LR Exposure Leads to Subfertility of Female Mice and Induces Oxidative Stress in Granulosa Cells
title_sort mc-lr exposure leads to subfertility of female mice and induces oxidative stress in granulosa cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690122/
https://www.ncbi.nlm.nih.gov/pubmed/26633508
http://dx.doi.org/10.3390/toxins7124872
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