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Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation
Radiation-induced genomic instability refers to a type of damage transmitted over many generations following irradiation. This delayed impact of radiation exposure may pose a high risk to human health and increases concern over the dose limit of radiation exposure for both the public and radiation w...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690915/ https://www.ncbi.nlm.nih.gov/pubmed/26633443 http://dx.doi.org/10.3390/ijerph121214979 |
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author | Cho, Yoon Hee Kim, Su Young Woo, Hae Dong Kim, Yang Jee Ha, Sung Whan Chung, Hai Won |
author_facet | Cho, Yoon Hee Kim, Su Young Woo, Hae Dong Kim, Yang Jee Ha, Sung Whan Chung, Hai Won |
author_sort | Cho, Yoon Hee |
collection | PubMed |
description | Radiation-induced genomic instability refers to a type of damage transmitted over many generations following irradiation. This delayed impact of radiation exposure may pose a high risk to human health and increases concern over the dose limit of radiation exposure for both the public and radiation workers. Therefore, the development of additional biomarkers is still needed for the detection of delayed responses following low doses of radiation exposure. In this study, we examined the effect of X-irradiation on delayed induction of numerical chromosomal aberrations in normal human fibroblasts irradiated with 20, 50 and 100 cGy of X-rays using the micronucleus-centromere assay. Frequencies of centromere negative- and positive-micronuclei, and aneuploidy of chromosome 1 and 4 were analyzed in the surviving cells at 28, 88 and 240 h after X-irradiation. X-irradiation increased the frequency of micronuclei (MN) in a dose-dependent manner in the cells at all measured time-points, but no significant differences in MN frequency among cell passages were observed. Aneuploid frequency of chromosomes 1 and 4 increased with radiation doses, and a significantly higher frequency of aneuploidy was observed in the surviving cells analyzed at 240 h compared to 28 h. These results indicate that low-dose of X-irradiation can induce delayed aneuploidy of chromosomes 1 and 4 in normal fibroblasts. |
format | Online Article Text |
id | pubmed-4690915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-46909152016-01-06 Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation Cho, Yoon Hee Kim, Su Young Woo, Hae Dong Kim, Yang Jee Ha, Sung Whan Chung, Hai Won Int J Environ Res Public Health Article Radiation-induced genomic instability refers to a type of damage transmitted over many generations following irradiation. This delayed impact of radiation exposure may pose a high risk to human health and increases concern over the dose limit of radiation exposure for both the public and radiation workers. Therefore, the development of additional biomarkers is still needed for the detection of delayed responses following low doses of radiation exposure. In this study, we examined the effect of X-irradiation on delayed induction of numerical chromosomal aberrations in normal human fibroblasts irradiated with 20, 50 and 100 cGy of X-rays using the micronucleus-centromere assay. Frequencies of centromere negative- and positive-micronuclei, and aneuploidy of chromosome 1 and 4 were analyzed in the surviving cells at 28, 88 and 240 h after X-irradiation. X-irradiation increased the frequency of micronuclei (MN) in a dose-dependent manner in the cells at all measured time-points, but no significant differences in MN frequency among cell passages were observed. Aneuploid frequency of chromosomes 1 and 4 increased with radiation doses, and a significantly higher frequency of aneuploidy was observed in the surviving cells analyzed at 240 h compared to 28 h. These results indicate that low-dose of X-irradiation can induce delayed aneuploidy of chromosomes 1 and 4 in normal fibroblasts. MDPI 2015-12-01 2015-12 /pmc/articles/PMC4690915/ /pubmed/26633443 http://dx.doi.org/10.3390/ijerph121214979 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cho, Yoon Hee Kim, Su Young Woo, Hae Dong Kim, Yang Jee Ha, Sung Whan Chung, Hai Won Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation |
title | Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation |
title_full | Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation |
title_fullStr | Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation |
title_full_unstemmed | Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation |
title_short | Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation |
title_sort | delayed numerical chromosome aberrations in human fibroblasts by low dose of radiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690915/ https://www.ncbi.nlm.nih.gov/pubmed/26633443 http://dx.doi.org/10.3390/ijerph121214979 |
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