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Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation

Radiation-induced genomic instability refers to a type of damage transmitted over many generations following irradiation. This delayed impact of radiation exposure may pose a high risk to human health and increases concern over the dose limit of radiation exposure for both the public and radiation w...

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Autores principales: Cho, Yoon Hee, Kim, Su Young, Woo, Hae Dong, Kim, Yang Jee, Ha, Sung Whan, Chung, Hai Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690915/
https://www.ncbi.nlm.nih.gov/pubmed/26633443
http://dx.doi.org/10.3390/ijerph121214979
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author Cho, Yoon Hee
Kim, Su Young
Woo, Hae Dong
Kim, Yang Jee
Ha, Sung Whan
Chung, Hai Won
author_facet Cho, Yoon Hee
Kim, Su Young
Woo, Hae Dong
Kim, Yang Jee
Ha, Sung Whan
Chung, Hai Won
author_sort Cho, Yoon Hee
collection PubMed
description Radiation-induced genomic instability refers to a type of damage transmitted over many generations following irradiation. This delayed impact of radiation exposure may pose a high risk to human health and increases concern over the dose limit of radiation exposure for both the public and radiation workers. Therefore, the development of additional biomarkers is still needed for the detection of delayed responses following low doses of radiation exposure. In this study, we examined the effect of X-irradiation on delayed induction of numerical chromosomal aberrations in normal human fibroblasts irradiated with 20, 50 and 100 cGy of X-rays using the micronucleus-centromere assay. Frequencies of centromere negative- and positive-micronuclei, and aneuploidy of chromosome 1 and 4 were analyzed in the surviving cells at 28, 88 and 240 h after X-irradiation. X-irradiation increased the frequency of micronuclei (MN) in a dose-dependent manner in the cells at all measured time-points, but no significant differences in MN frequency among cell passages were observed. Aneuploid frequency of chromosomes 1 and 4 increased with radiation doses, and a significantly higher frequency of aneuploidy was observed in the surviving cells analyzed at 240 h compared to 28 h. These results indicate that low-dose of X-irradiation can induce delayed aneuploidy of chromosomes 1 and 4 in normal fibroblasts.
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spelling pubmed-46909152016-01-06 Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation Cho, Yoon Hee Kim, Su Young Woo, Hae Dong Kim, Yang Jee Ha, Sung Whan Chung, Hai Won Int J Environ Res Public Health Article Radiation-induced genomic instability refers to a type of damage transmitted over many generations following irradiation. This delayed impact of radiation exposure may pose a high risk to human health and increases concern over the dose limit of radiation exposure for both the public and radiation workers. Therefore, the development of additional biomarkers is still needed for the detection of delayed responses following low doses of radiation exposure. In this study, we examined the effect of X-irradiation on delayed induction of numerical chromosomal aberrations in normal human fibroblasts irradiated with 20, 50 and 100 cGy of X-rays using the micronucleus-centromere assay. Frequencies of centromere negative- and positive-micronuclei, and aneuploidy of chromosome 1 and 4 were analyzed in the surviving cells at 28, 88 and 240 h after X-irradiation. X-irradiation increased the frequency of micronuclei (MN) in a dose-dependent manner in the cells at all measured time-points, but no significant differences in MN frequency among cell passages were observed. Aneuploid frequency of chromosomes 1 and 4 increased with radiation doses, and a significantly higher frequency of aneuploidy was observed in the surviving cells analyzed at 240 h compared to 28 h. These results indicate that low-dose of X-irradiation can induce delayed aneuploidy of chromosomes 1 and 4 in normal fibroblasts. MDPI 2015-12-01 2015-12 /pmc/articles/PMC4690915/ /pubmed/26633443 http://dx.doi.org/10.3390/ijerph121214979 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cho, Yoon Hee
Kim, Su Young
Woo, Hae Dong
Kim, Yang Jee
Ha, Sung Whan
Chung, Hai Won
Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation
title Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation
title_full Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation
title_fullStr Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation
title_full_unstemmed Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation
title_short Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation
title_sort delayed numerical chromosome aberrations in human fibroblasts by low dose of radiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690915/
https://www.ncbi.nlm.nih.gov/pubmed/26633443
http://dx.doi.org/10.3390/ijerph121214979
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