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Adenylylation of small RNA sequencing adapters using the TS2126 RNA ligase I
Many high-throughput small RNA next-generation sequencing protocols use 5′ preadenylylated DNA oligonucleotide adapters during cDNA library preparation. Preadenylylation of the DNA adapter's 5′ end frees from ATP-dependence the ligation of the adapter to RNA collections, thereby avoiding ATP-de...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Cold Spring Harbor Laboratory Press
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691829/ https://www.ncbi.nlm.nih.gov/pubmed/26567315 http://dx.doi.org/10.1261/rna.054999.115 |