High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Escherichia coli (E. coli) is the most widely used expression system for the production of recombinant proteins for structural and functional studies. However, purifying proteins is sometimes challenging since many proteins are expressed in an insoluble form. When working with difficult or multiple...

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Detalles Bibliográficos
Autores principales: Saez, Natalie J., Nozach, Hervé, Blemont, Marilyne, Vincentelli, Renaud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2014
Materias:
Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692350/
https://www.ncbi.nlm.nih.gov/pubmed/25146501
http://dx.doi.org/10.3791/51464

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692350/
https://www.ncbi.nlm.nih.gov/pubmed/25146501
http://dx.doi.org/10.3791/51464

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