Cargando…

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Escherichia coli (E. coli) is the most widely used expression system for the production of recombinant proteins for structural and functional studies. However, purifying proteins is sometimes challenging since many proteins are expressed in an insoluble form. When working with difficult or multiple...

Descripción completa

Detalles Bibliográficos
Autores principales:	Saez, Natalie J., Nozach, Hervé, Blemont, Marilyne, Vincentelli, Renaud
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2014
Materias:	Bioengineering
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692350/ https://www.ncbi.nlm.nih.gov/pubmed/25146501 http://dx.doi.org/10.3791/51464

Ejemplares similares

High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli
por: Nozach, Hervé, et al.
Publicado: (2013)

Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli
por: Sequeira, Ana Filipa, et al.
Publicado: (2017)

High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery
por: Turchetto, Jeremy, et al.
Publicado: (2017)

Production of Recombinant Disulfide-Rich Venom Peptides for Structural and Functional Analysis via Expression in the Periplasm of E. coli
por: Klint, Julie K., et al.
Publicado: (2013)

Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins
por: Rivera-de-Torre, Esperanza, et al.
Publicado: (2022)

The deep-rooted origin of disulfide-rich spider venom toxins
por: Shaikh, Naeem Yusuf, et al.
Publicado: (2023)

Molecular Simulations of Disulfide-Rich Venom Peptides with Ion Channels and Membranes
por: Deplazes, Evelyne
Publicado: (2017)

Quantifying domain-ligand affinities and specificities by high-throughput holdup assay
por: Vincentelli, Renaud, et al.
Publicado: (2015)

Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
por: Estrada-Gomez, Sebastian, et al.
Publicado: (2019)

High-throughput Purification of Affinity-tagged Recombinant Proteins
por: Wiesler, Simone C., et al.
Publicado: (2012)

Expression and Purification of Recombinant Hemoglobin in Escherichia coli
por: Natarajan, Chandrasekhar, et al.
Publicado: (2011)

Development of a Metabolite Sensor for High-Throughput Detection of Aldehydes in Escherichia Coli
por: Frazão, Cláudio R., et al.
Publicado: (2018)

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
por: Garcia-Schwarz, Giancarlo, et al.
Publicado: (2012)

Preparation, Purification, and Use of Fatty Acid-containing Liposomes
por: Jin, Lin, et al.
Publicado: (2018)

Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli
por: Karyolaimos, Alexandros, et al.
Publicado: (2021)

Recombinant expression and downstream processing of the disulfide-rich tumor-targeting peptide chlorotoxin
por: WANG, XIAO-MIN, et al.
Publicado: (2013)

Cloning, expression and purification of recombinant dermatopontin in Escherichia coli
por: Seetaraman Amritha, Trikkur Madom, et al.
Publicado: (2020)

FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult Recombinant Proteins in Drosophila S2 Cells
por: Coker, Jesse A., et al.
Publicado: (2022)

High-Throughput Venomics
por: Slagboom, Julien, et al.
Publicado: (2023)

Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli
por: de Marco, Ario
Publicado: (2009)

Polymer Microarrays for High Throughput Discovery of Biomaterials
por: Hook, Andrew L., et al.
Publicado: (2012)

Recombinant production, purification, and biochemical characterization of a novel L-lactate dehydrogenase from Bacillus cereus NRC1 and inhibition study of mangiferin
por: Esa, Sayed S., et al.
Publicado: (2023)

Improvement of the Stability and Activity of an LPMO Through Rational Disulfide Bonds Design
por: Zhou, Xiaoli, et al.
Publicado: (2022)

A positive Selection Escherichia Coli Recombinant Protein Expression Vector for One-Step Cloning
por: James, Shinto, et al.
Publicado: (2022)

Optimization of Culture Conditions for Secretory Production of 3-Hydroxybutyrate Oligomers Using Recombinant Escherichia coli
por: Sakurai, Tetsuo, et al.
Publicado: (2022)

Optimisation of recombinant TNFα production in Escherichia coli using GFP fusions and flow cytometry
por: Zulkifly, Nurul Asma Hasliza, et al.
Publicado: (2023)

Soluble Expression of Human Leukemia Inhibitory Factor with Protein Disulfide Isomerase in Escherichia coli and Its Simple Purification
por: Jung, A. Song, et al.
Publicado: (2013)

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
por: Demaree, Benjamin, et al.
Publicado: (2018)

Methylase-assisted subcloning for high throughput BioBrick assembly
por: Matsumura, Ichiro
Publicado: (2020)

Selection of High Producers From Combinatorial Libraries for the Production of Recombinant Proteins in Escherichia coli and Vibrio natriegens
por: Eichmann, Joel, et al.
Publicado: (2019)

Escherichia coli Can Adapt Its Protein Translocation Machinery for Enhanced Periplasmic Recombinant Protein Production
por: Karyolaimos, Alexandros, et al.
Publicado: (2020)

Sample Limited Characterization of a Novel Disulfide-Rich Venom Peptide Toxin from Terebrid Marine Snail Terebra variegata
por: Anand, Prachi, et al.
Publicado: (2014)

Homologous high-throughput expression and purification of highly conserved E coli proteins
por: Ergin, Asgar, et al.
Publicado: (2007)

Shikimic Acid Production in Escherichia coli: From Classical Metabolic Engineering Strategies to Omics Applied to Improve Its Production
por: Martínez, Juan Andrés, et al.
Publicado: (2015)

Single-Input Regulatory Cascade for in vivo Removal of the Solubility Tag in Fusion Recombinant Proteins Produced by Escherichia coli
por: Silva, Filipe S. R., et al.
Publicado: (2019)

Selective Release of Recombinant Periplasmic Protein From E. coli Using Continuous Pulsed Electric Field Treatment
por: Schottroff, Felix, et al.
Publicado: (2021)

Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications
por: Bhatwa, Arshpreet, et al.
Publicado: (2021)

Review on Strategies and Technologies for Exosome Isolation and Purification
por: Chen, Jiaci, et al.
Publicado: (2022)

Empowering a Methanol-Dependent Escherichia coli via Adaptive Evolution Using a High-Throughput Microbial Microdroplet Culture System
por: Wang, Jia, et al.
Publicado: (2020)

Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification
por: Shankar, Ram, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_cja4erbqd1modkqadb23t06t8j