Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

MscL, a large conductance mechanosensitive channel (MSC), is a ubiquitous osmolyte release valve that helps bacteria survive abrupt hypo-osmotic shocks. It has been discovered and rigorously studied using the patch-clamp technique for almost three decades. Its basic role of translating tension appli...

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Detalles Bibliográficos
Autores principales: Najem, Joseph S., Dunlap, Myles D., Yasmann, Anthony, Freeman, Eric C., Grant, John W., Sukharev, Sergei, Leo, Donald J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692740/
https://www.ncbi.nlm.nih.gov/pubmed/26650467
http://dx.doi.org/10.3791/53362

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692740/
https://www.ncbi.nlm.nih.gov/pubmed/26650467
http://dx.doi.org/10.3791/53362

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