Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

We have developed a protocol improving current Embryoid Body (EB) culture which allows the study of self-organization, symmetry breaking, axial elongation and cell fate specification using aggregates of mouse embryonic stem cells (mESCs) in suspension culture. Small numbers of mESCs are aggregated i...

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Detalles Bibliográficos
Autores principales: Baillie-Johnson, Peter, van den Brink, Susanne Carina, Balayo, Tina, Turner, David Andrew, Martinez Arias, Alfonso
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692741/
https://www.ncbi.nlm.nih.gov/pubmed/26650833
http://dx.doi.org/10.3791/53252

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692741/
https://www.ncbi.nlm.nih.gov/pubmed/26650833
http://dx.doi.org/10.3791/53252

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