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Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells
Rybp (Ring1 and Yy1 Binding Protein) is a transcriptional regulator and member of the noncanonical polycomb repressive complex 1 with essential role in early embryonic development. We have previously described that alteration of Rybp dosage in mouse models induced striking neural tube defects (NTDs)...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4693026/ https://www.ncbi.nlm.nih.gov/pubmed/26788067 http://dx.doi.org/10.1155/2016/4034620 |
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author | Kovacs, Gergo Szabo, Viktoria Pirity, Melinda K. |
author_facet | Kovacs, Gergo Szabo, Viktoria Pirity, Melinda K. |
author_sort | Kovacs, Gergo |
collection | PubMed |
description | Rybp (Ring1 and Yy1 Binding Protein) is a transcriptional regulator and member of the noncanonical polycomb repressive complex 1 with essential role in early embryonic development. We have previously described that alteration of Rybp dosage in mouse models induced striking neural tube defects (NTDs), exencephaly, and disorganized neurocortex. In this study we further investigated the role of Rybp in neural differentiation by utilising wild type (rybp (+/+)) and rybp null mutant (rybp (−/−)) embryonic stem cells (ESCs) and tried to uncover underlying molecular events that are responsible for the observed phenotypic changes. We found that rybp null mutant ESCs formed less matured neurons, astrocytes, and oligodendrocytes from existing progenitors than wild type cells. Furthermore, lack of rybp coincided with altered gene expression of key neural markers including Pax6 and Plagl1 pinpointing a possible transcriptional circuit among these genes. |
format | Online Article Text |
id | pubmed-4693026 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-46930262016-01-19 Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells Kovacs, Gergo Szabo, Viktoria Pirity, Melinda K. Stem Cells Int Research Article Rybp (Ring1 and Yy1 Binding Protein) is a transcriptional regulator and member of the noncanonical polycomb repressive complex 1 with essential role in early embryonic development. We have previously described that alteration of Rybp dosage in mouse models induced striking neural tube defects (NTDs), exencephaly, and disorganized neurocortex. In this study we further investigated the role of Rybp in neural differentiation by utilising wild type (rybp (+/+)) and rybp null mutant (rybp (−/−)) embryonic stem cells (ESCs) and tried to uncover underlying molecular events that are responsible for the observed phenotypic changes. We found that rybp null mutant ESCs formed less matured neurons, astrocytes, and oligodendrocytes from existing progenitors than wild type cells. Furthermore, lack of rybp coincided with altered gene expression of key neural markers including Pax6 and Plagl1 pinpointing a possible transcriptional circuit among these genes. Hindawi Publishing Corporation 2016 2015-12-15 /pmc/articles/PMC4693026/ /pubmed/26788067 http://dx.doi.org/10.1155/2016/4034620 Text en Copyright © 2016 Gergo Kovacs et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kovacs, Gergo Szabo, Viktoria Pirity, Melinda K. Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells |
title | Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells |
title_full | Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells |
title_fullStr | Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells |
title_full_unstemmed | Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells |
title_short | Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells |
title_sort | absence of rybp compromises neural differentiation of embryonic stem cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4693026/ https://www.ncbi.nlm.nih.gov/pubmed/26788067 http://dx.doi.org/10.1155/2016/4034620 |
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