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RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences

The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown. In one of these, an external guide sequence, which mimic...

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Autores principales: Derksen, Merel, Mertens, Vicky, Pruijn, Ger J.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4693268/
https://www.ncbi.nlm.nih.gov/pubmed/26569326
http://dx.doi.org/10.3390/biom5043029
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author Derksen, Merel
Mertens, Vicky
Pruijn, Ger J.M.
author_facet Derksen, Merel
Mertens, Vicky
Pruijn, Ger J.M.
author_sort Derksen, Merel
collection PubMed
description The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown. In one of these, an external guide sequence, which mimics a tRNA precursor, a well-known natural RNase P substrate, is used to target an RNA molecule for cleavage by endogenous RNase P. Alternatively, a guide sequence can be attached to M1 RNA, the (catalytic) RNase P RNA subunit of Escherichia coli. The guide sequence is specific for an RNA target, which is subsequently cleaved by the bacterial M1 RNA moiety. These approaches are applicable in both bacteria and eukaryotes. In this review, we will discuss the two technologies in which RNase P is used to reduce RNA expression levels.
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spelling pubmed-46932682016-01-07 RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences Derksen, Merel Mertens, Vicky Pruijn, Ger J.M. Biomolecules Review The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown. In one of these, an external guide sequence, which mimics a tRNA precursor, a well-known natural RNase P substrate, is used to target an RNA molecule for cleavage by endogenous RNase P. Alternatively, a guide sequence can be attached to M1 RNA, the (catalytic) RNase P RNA subunit of Escherichia coli. The guide sequence is specific for an RNA target, which is subsequently cleaved by the bacterial M1 RNA moiety. These approaches are applicable in both bacteria and eukaryotes. In this review, we will discuss the two technologies in which RNase P is used to reduce RNA expression levels. MDPI 2015-11-09 /pmc/articles/PMC4693268/ /pubmed/26569326 http://dx.doi.org/10.3390/biom5043029 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Derksen, Merel
Mertens, Vicky
Pruijn, Ger J.M.
RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences
title RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences
title_full RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences
title_fullStr RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences
title_full_unstemmed RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences
title_short RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences
title_sort rnase p-mediated sequence-specific cleavage of rna by engineered external guide sequences
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4693268/
https://www.ncbi.nlm.nih.gov/pubmed/26569326
http://dx.doi.org/10.3390/biom5043029
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