A Streamlined, Automated Protocol for the Production of Milligram Quantities of Untagged Recombinant Rat Lactate Dehydrogenase A Using ÄKTAxpress(TM)

We developed an efficient, automated 2-step purification protocol for the production of milligram quantities of untagged recombinant rat lactate dehydrogenase A (rLDHA) from E. coli, using the ÄKTAxpress™ chromatography system. Cation exchange followed by size exclusion results in average final puri...

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Detalles Bibliográficos
Autores principales: Nowicki, Matthew W., Blackburn, Elizabeth A., McNae, Iain W., Wear, Martin A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696747/
https://www.ncbi.nlm.nih.gov/pubmed/26717415
http://dx.doi.org/10.1371/journal.pone.0146164

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696747/
https://www.ncbi.nlm.nih.gov/pubmed/26717415
http://dx.doi.org/10.1371/journal.pone.0146164

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