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Rational Design of High-Number dsDNA Fragments Based on Thermodynamics for the Construction of Full-Length Genes in a Single Reaction

Gene synthesis is frequently used in modern molecular biology research either to create novel genes or to obtain natural genes when the synthesis approach is more flexible and reliable than cloning. DNA chemical synthesis has limits on both its length and yield, thus full-length genes have to be hie...

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Detalles Bibliográficos
Autores principales: Birla, Bhagyashree S., Chou, Hui-Hsien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696799/
https://www.ncbi.nlm.nih.gov/pubmed/26716828
http://dx.doi.org/10.1371/journal.pone.0145682