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Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae
Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at endogenous levels. This is especially useful for incor...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4699809/ https://www.ncbi.nlm.nih.gov/pubmed/26727004 http://dx.doi.org/10.1371/journal.pone.0146120 |