Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae

Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at endogenous levels. This is especially useful for incor...

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Detalles Bibliográficos
Autores principales: Higuchi-Sanabria, Ryo, Garcia, Enrique J., Tomoiaga, Delia, Munteanu, Emilia L., Feinstein, Paul, Pon, Liza A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4699809/
https://www.ncbi.nlm.nih.gov/pubmed/26727004
http://dx.doi.org/10.1371/journal.pone.0146120
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author Higuchi-Sanabria, Ryo
Garcia, Enrique J.
Tomoiaga, Delia
Munteanu, Emilia L.
Feinstein, Paul
Pon, Liza A.
author_facet Higuchi-Sanabria, Ryo
Garcia, Enrique J.
Tomoiaga, Delia
Munteanu, Emilia L.
Feinstein, Paul
Pon, Liza A.
author_sort Higuchi-Sanabria, Ryo
collection PubMed
description Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at endogenous levels. This is especially useful for incorporation of multiple fluorescent protein fusions into a single strain, which can be challenging in organisms where genetic manipulation is more complex. However, the availability of optimal fluorescent protein combinations for 3-color imaging is limited. Here, we have characterized a combination of fluorescent proteins, mTFP1/mCitrine/mCherry for multicolor live cell imaging in S. cerevisiae. This combination can be used with conventional blue dyes, such as DAPI, for potential four-color live cell imaging.
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spelling pubmed-46998092016-01-15 Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae Higuchi-Sanabria, Ryo Garcia, Enrique J. Tomoiaga, Delia Munteanu, Emilia L. Feinstein, Paul Pon, Liza A. PLoS One Research Article Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at endogenous levels. This is especially useful for incorporation of multiple fluorescent protein fusions into a single strain, which can be challenging in organisms where genetic manipulation is more complex. However, the availability of optimal fluorescent protein combinations for 3-color imaging is limited. Here, we have characterized a combination of fluorescent proteins, mTFP1/mCitrine/mCherry for multicolor live cell imaging in S. cerevisiae. This combination can be used with conventional blue dyes, such as DAPI, for potential four-color live cell imaging. Public Library of Science 2016-01-04 /pmc/articles/PMC4699809/ /pubmed/26727004 http://dx.doi.org/10.1371/journal.pone.0146120 Text en © 2016 Higuchi-Sanabria et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Research Article
Higuchi-Sanabria, Ryo
Garcia, Enrique J.
Tomoiaga, Delia
Munteanu, Emilia L.
Feinstein, Paul
Pon, Liza A.
Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae
title Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae
title_full Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae
title_fullStr Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae
title_full_unstemmed Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae
title_short Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae
title_sort characterization of fluorescent proteins for three- and four-color live-cell imaging in s. cerevisiae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4699809/
https://www.ncbi.nlm.nih.gov/pubmed/26727004
http://dx.doi.org/10.1371/journal.pone.0146120
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