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Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae

Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at endogenous levels. This is especially useful for incor...

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Detalles Bibliográficos
Autores principales:	Higuchi-Sanabria, Ryo, Garcia, Enrique J., Tomoiaga, Delia, Munteanu, Emilia L., Feinstein, Paul, Pon, Liza A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2016
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4699809/ https://www.ncbi.nlm.nih.gov/pubmed/26727004 http://dx.doi.org/10.1371/journal.pone.0146120

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