Promotion of Bamboo Mosaic Virus Accumulation in Nicotiana benthamiana by 5′→3′ Exonuclease NbXRN4

Bamboo mosaic virus (BaMV) has a 6.4-kb (+) sense RNA genome with a 5′ cap and a 3′ poly(A) tail. ORF1 of this potexvirus encodes a 155-kDa replication protein responsible for the viral RNA replication/transcription and 5′ cap formation. To learn more about the replication complex of BaMV, a protein...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Cheng-Cheng, Lin, Tzu-Ling, Lin, Jhe-Wei, Han, Yu-Tsung, Huang, Yu-Ting, Hsu, Yau-Heiu, Meng, Menghsiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4702010/
https://www.ncbi.nlm.nih.gov/pubmed/26779163
http://dx.doi.org/10.3389/fmicb.2015.01508
_version_ 1782408573286875136
author Lee, Cheng-Cheng
Lin, Tzu-Ling
Lin, Jhe-Wei
Han, Yu-Tsung
Huang, Yu-Ting
Hsu, Yau-Heiu
Meng, Menghsiao
author_facet Lee, Cheng-Cheng
Lin, Tzu-Ling
Lin, Jhe-Wei
Han, Yu-Tsung
Huang, Yu-Ting
Hsu, Yau-Heiu
Meng, Menghsiao
author_sort Lee, Cheng-Cheng
collection PubMed
description Bamboo mosaic virus (BaMV) has a 6.4-kb (+) sense RNA genome with a 5′ cap and a 3′ poly(A) tail. ORF1 of this potexvirus encodes a 155-kDa replication protein responsible for the viral RNA replication/transcription and 5′ cap formation. To learn more about the replication complex of BaMV, a protein preparation enriched in the 155-kDa replication protein was obtained from Nicotiana benthamiana by a protocol involving agroinfiltration and immunoprecipitation. Subsequent analysis by SDS-PAGE and mass spectrometry identified a handful of host proteins that may participate in the viral replication. Among them, the cytoplasmic exoribonuclease NbXRN4 particularly caught our attention. NbXRN4 has been shown to have an antiviral activity against Tomato bushy stunt virus and Tomato mosaic virus. In Arabidopsis, the enzyme could reduce RNAi- and miRNA-mediated RNA decay. This study found that downregulation of NbXRN4 greatly decreased BaMV accumulation, while overexpression of NbXRN4 resulted in an opposite effect. Mutations at the catalytically essential residues abolished the function of NbXRN4 in the increase of BaMV accumulation. Nonetheless, NbXRN4 was still able to promote BaMV accumulation in the presence of the RNA silencing suppressor P19. In summary, the replication efficiency of BaMV may be improved by the exoribonuclease activity of NbXRN4.
format Online
Article
Text
id pubmed-4702010
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-47020102016-01-15 Promotion of Bamboo Mosaic Virus Accumulation in Nicotiana benthamiana by 5′→3′ Exonuclease NbXRN4 Lee, Cheng-Cheng Lin, Tzu-Ling Lin, Jhe-Wei Han, Yu-Tsung Huang, Yu-Ting Hsu, Yau-Heiu Meng, Menghsiao Front Microbiol Microbiology Bamboo mosaic virus (BaMV) has a 6.4-kb (+) sense RNA genome with a 5′ cap and a 3′ poly(A) tail. ORF1 of this potexvirus encodes a 155-kDa replication protein responsible for the viral RNA replication/transcription and 5′ cap formation. To learn more about the replication complex of BaMV, a protein preparation enriched in the 155-kDa replication protein was obtained from Nicotiana benthamiana by a protocol involving agroinfiltration and immunoprecipitation. Subsequent analysis by SDS-PAGE and mass spectrometry identified a handful of host proteins that may participate in the viral replication. Among them, the cytoplasmic exoribonuclease NbXRN4 particularly caught our attention. NbXRN4 has been shown to have an antiviral activity against Tomato bushy stunt virus and Tomato mosaic virus. In Arabidopsis, the enzyme could reduce RNAi- and miRNA-mediated RNA decay. This study found that downregulation of NbXRN4 greatly decreased BaMV accumulation, while overexpression of NbXRN4 resulted in an opposite effect. Mutations at the catalytically essential residues abolished the function of NbXRN4 in the increase of BaMV accumulation. Nonetheless, NbXRN4 was still able to promote BaMV accumulation in the presence of the RNA silencing suppressor P19. In summary, the replication efficiency of BaMV may be improved by the exoribonuclease activity of NbXRN4. Frontiers Media S.A. 2016-01-06 /pmc/articles/PMC4702010/ /pubmed/26779163 http://dx.doi.org/10.3389/fmicb.2015.01508 Text en Copyright © 2016 Lee, Lin, Lin, Han, Huang, Hsu and Meng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lee, Cheng-Cheng
Lin, Tzu-Ling
Lin, Jhe-Wei
Han, Yu-Tsung
Huang, Yu-Ting
Hsu, Yau-Heiu
Meng, Menghsiao
Promotion of Bamboo Mosaic Virus Accumulation in Nicotiana benthamiana by 5′→3′ Exonuclease NbXRN4
title Promotion of Bamboo Mosaic Virus Accumulation in Nicotiana benthamiana by 5′→3′ Exonuclease NbXRN4
title_full Promotion of Bamboo Mosaic Virus Accumulation in Nicotiana benthamiana by 5′→3′ Exonuclease NbXRN4
title_fullStr Promotion of Bamboo Mosaic Virus Accumulation in Nicotiana benthamiana by 5′→3′ Exonuclease NbXRN4
title_full_unstemmed Promotion of Bamboo Mosaic Virus Accumulation in Nicotiana benthamiana by 5′→3′ Exonuclease NbXRN4
title_short Promotion of Bamboo Mosaic Virus Accumulation in Nicotiana benthamiana by 5′→3′ Exonuclease NbXRN4
title_sort promotion of bamboo mosaic virus accumulation in nicotiana benthamiana by 5′→3′ exonuclease nbxrn4
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4702010/
https://www.ncbi.nlm.nih.gov/pubmed/26779163
http://dx.doi.org/10.3389/fmicb.2015.01508
work_keys_str_mv AT leechengcheng promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4
AT lintzuling promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4
AT linjhewei promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4
AT hanyutsung promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4
AT huangyuting promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4
AT hsuyauheiu promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4
AT mengmenghsiao promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4