A generic strategy for CRISPR-Cas9-mediated gene tagging

Genome engineering has been greatly enhanced by the availability of Cas9 endonuclease that can be targeted to almost any genomic locus using so called guide RNAs (gRNAs). However, the introduction of foreign DNA sequences to tag an endogenous gene is still cumbersome as it requires the synthesis or...

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Detalles Bibliográficos
Autores principales: Lackner, Daniel H., Carré, Alexia, Guzzardo, Paloma M., Banning, Carina, Mangena, Ramu, Henley, Tom, Oberndorfer, Sarah, Gapp, Bianca V., Nijman, Sebastian M.B., Brummelkamp, Thijn R., Bürckstümmer, Tilmann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4703899/
https://www.ncbi.nlm.nih.gov/pubmed/26674669
http://dx.doi.org/10.1038/ncomms10237

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4703899/
https://www.ncbi.nlm.nih.gov/pubmed/26674669
http://dx.doi.org/10.1038/ncomms10237

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