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Comparing the Affinity of GTPase-binding Proteins using Competition Assays
In this protocol we demonstrate a method for comparing the competition between GTPase-binding proteins. Such an approach is important for determining the binding capabilities of GTPases for two reasons: The fact that all interactions involve the same face of the GTPases means that binding events mus...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MyJove Corporation
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4705812/ https://www.ncbi.nlm.nih.gov/pubmed/26484896 http://dx.doi.org/10.3791/53254 |