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Development and Application of a High Throughput Protein Unfolding Kinetic Assay

The kinetics of folding and unfolding underlie protein stability and quantification of these rates provides important insights into the folding process. Here, we present a simple high throughput protein unfolding kinetic assay using a plate reader that is applicable to the studies of the majority of...

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Detalles Bibliográficos
Autores principales: Wang, Qiang, Waterhouse, Nicklas, Feyijinmi, Olusegun, Dominguez, Matthew J., Martinez, Lisa M., Sharp, Zoey, Service, Rachel, Bothe, Jameson R., Stollar, Elliott J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4706425/
https://www.ncbi.nlm.nih.gov/pubmed/26745729
http://dx.doi.org/10.1371/journal.pone.0146232