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Influence of a family 29 carbohydrate binding module on the recombinant production of galactose oxidase in Pichia pastoris

Herein, we report the extracellular expression of carbohydrate active fusion enzymes in Pichia pastoris. Particularly, CBM29-1-2 from Piromyces equi was separately fused to the N- and C-terminus of galactose 6-oxidase (GaO, D-galactose: oxygen 6-oxidoreductase, EC 1.1.13.9, CAZy family AA5) from Fus...

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Detalles Bibliográficos
Autores principales: Mollerup, Filip, Master, Emma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4706568/
https://www.ncbi.nlm.nih.gov/pubmed/26858983
http://dx.doi.org/10.1016/j.dib.2015.11.032