Digital PCR for direct quantification of viruses without DNA extraction

DNA extraction before amplification is considered an essential step for quantification of viral DNA using real-time PCR (qPCR). However, this can directly affect the final measurements due to variable DNA yields and removal of inhibitors, which leads to increased inter-laboratory variability of qPCR...

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Detalles Bibliográficos
Autores principales: Pavšič, Jernej, Žel, Jana, Milavec, Mojca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4706837/
https://www.ncbi.nlm.nih.gov/pubmed/26483186
http://dx.doi.org/10.1007/s00216-015-9109-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4706837/
https://www.ncbi.nlm.nih.gov/pubmed/26483186
http://dx.doi.org/10.1007/s00216-015-9109-0

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